Journal of Dermatological Science
Volume 16, Issue 3 , Pages 226-230, March 1998

Modulation of UVA light-induced oxidative stress by β-carotene, lutein and astaxanthin in cultured fibroblasts

Department of Nutrition, National Food Biotechnology Centre, University College, Cork, Ireland

Received 14 April 1997; received in revised form 16 June 1997; accepted 8 July 1997.

Abstract 

The ability of β-carotene, lutein or astaxanthin to protect against UVA-induced oxidative stress in rat kidney fibroblasts (NRK) was assessed. Activities of the antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD), and changes in thiobarbituric acid reactive substances (TBARS) were measured as indices of oxidative stress. Exposure to UVA light at a dose intensity of 5.6 mW/cm2 for 4 h resulted in a significant decrease in CAT and SOD activities and a significant increase in TBARS. No cytotoxicity, as indicated by lactate dehydrogenase (LDH) release, was observed. β-Carotene (1 μM), lutein (1 μM) and astaxanthin (10 nM) protect against UVA light-induced oxidative stress in vitro with astaxanthin exhibiting superior protective properties.

Keywords:  UVA light, β-Carotene, Lutein, Astaxanthin, Rat kidney fibroblasts

To access this article, please choose from the options below

Login to an existing account or Register a new account.

  • Purchase this article for 31.50 USD (You must login/register to purchase this article)

    Online access for 24 hours. The PDF version can be downloaded as your permanent record.

  • Claim access now

    For current subscribers with Society Membership or Account Number.

  • Visit SciVerse ScienceDirect to see if you have access via your institution.
 

PII: S0923-1811(97)00058-3

Journal of Dermatological Science
Volume 16, Issue 3 , Pages 226-230, March 1998

Article Tools