« Back Journal of Dermatological Science

Article in Press

Cutaneous consequences of inhibiting EGF receptor signaling in vivo: Normal hair follicle development, but retarded hair cycle induction and inhibition of adipocyte growth in EgfrWa5 mice

  • Koji Sugawara

      Affiliations

    • Department of Dermatology, University of Lübeck, Lübeck, Germany
    • These authors contributed equally to this work.
  • ,
  • Marlon R. Schneider

      Affiliations

    • Institute of Molecular Animal Breeding and Biotechnology, Gene Center, LMU Munich, Feodor-Lynen-Str. 25, 81377 Munich, Germany
    • These authors contributed equally to this work.
    • Corresponding Author InformationCorresponding author. Tel.: +49 89 218076815.
  • ,
  • Maik Dahlhoff

      Affiliations

    • Institute of Molecular Animal Breeding and Biotechnology, Gene Center, LMU Munich, Feodor-Lynen-Str. 25, 81377 Munich, Germany
  • ,
  • Jennifer E. Kloepper

      Affiliations

    • Institute of Molecular Animal Breeding and Biotechnology, Gene Center, LMU Munich, Feodor-Lynen-Str. 25, 81377 Munich, Germany
  • ,
  • Ralf Paus

      Affiliations

    • Department of Dermatology, University of Lübeck, Lübeck, Germany
    • School of Translational Medicine, University of Manchester, Manchester, UK

Received 24 June 2009 ,Revised 19 November 2009 ,Accepted 7 December 2009. Published online 12 July 2010 Corrected Proof

References 

  1. Schneider MR, Wolf E. The epidermal growth factor receptor ligands at a glance. J Cell Physiol. 2009;218:460–466
  2. Yarden Y, Sliwkowski MX. Untangling the ErbB signalling network. Nat Rev Mol Cell Biol. 2001;2:127–137
  3. Citri A, Yarden Y. EGF-ERBB signalling: towards the systems level. Nat Rev Mol Cell Biol. 2006;7:505–516
  4. Yarden Y, Shilo BZ, SnapShot: . EGFR signaling pathway. Cell. 2007;131:1018
  5. Pastore S, Mascia F, Mariani V, Girolomoni G. The epidermal growth factor receptor system in skin repair and inflammation. J Invest Dermatol. 2008;128:1365–1374
  6. Schneider MR, Werner S, Paus R, Wolf E. Beyond wavy hairs: the epidermal growth factor receptor and its ligands in skin biology and pathology. Am J Pathol. 2008;173:14–24
  7. Keeler CE. A second rexoid coat character in the house mouse. J Hered. 1935;26:189–191
  8. Luetteke NC, Phillips HK, Qiu TH, Copeland NG, Earp HS, Jenkins NA, et al. The mouse waved-2 phenotype results from a point mutation in the EGF receptor tyrosine kinase. Genes Dev. 1994;8:399–413
  9. Fowler KJ, Walker F, Alexander W, Hibbs ML, Nice EC, Bohmer RM, et al. A mutation in the epidermal growth factor receptor in waved-2 mice has a profound effect on receptor biochemistry that results in impaired lactation. Proc Natl Acad Sci USA. 1995;92:1465–1469
  10. Threadgill DW, Dlugosz AA, Hansen LA, Tennenbaum T, Lichti U, Yee D, et al. Targeted disruption of mouse EGF receptor: effect of genetic background on mutant phenotype. Science. 1995;269:230–234
  11. Sibilia M, Wagner EF. Strain-dependent epithelial defects in mice lacking the EGF receptor. Science. 1995;269:234–238
  12. Miettinen PJ, Berger JE, Meneses J, Phung Y, Pedersen RA, Werb Z, et al. Epithelial immaturity and multiorgan failure in mice lacking epidermal growth factor receptor. Nature. 1995;376:337–341
  13. Hansen LA, Alexander N, Hogan ME, Sundberg JP, Dlugosz A, Threadgill DW, et al. Genetically null mice reveal a central role for epidermal growth factor receptor in the differentiation of the hair follicle and normal hair development. Am J Pathol. 1997;150:1959–1975
  14. Sibilia M, Wagner B, Hoebertz A, Elliott C, Marino S, Jochum W, et al. Mice humanised for the EGF receptor display hypomorphic phenotypes in skin, bone and heart. Development. 2003;130:4515–4525
  15. Thaung C, West K, Clark BJ, McKie L, Morgan JE, Arnold K, et al. Novel ENU-induced eye mutations in the mouse: models for human eye disease. Hum Mol Genet. 2002;11:755–767
  16. Lee D, Cross SH, Strunk KE, Morgan JE, Bailey CL, Jackson IJ, et al. Wa5 is a novel ENU-induced antimorphic allele of the epidermal growth factor receptor. Mamm Genome. 2004;15:525–536
  17. Du X, Tabeta K, Hoebe K, Liu H, Mann N, Mudd S, et al. Velvet, a dominant Egfr mutation that causes wavy hair and defective eyelid development in mice. Genetics. 2004;166:331–340
  18. Fitch KR, McGowan KA, van Raamsdonk CD, Fuchs H, Lee D, Puech A, et al. Genetics of dark skin in mice. Genes Dev. 2003;17:214–228
  19. Murillas R, Larcher F, Conti CJ, Santos M, Ullrich A, Jorcano JL. Expression of a dominant negative mutant of epidermal growth factor receptor in the epidermis of transgenic mice elicits striking alterations in hair follicle development and skin structure. EMBO J. 1995;14:5216–5223
  20. Paus R, Muller-Rover S, Van DV, Maurer M, Eichmuller S, Ling G, et al. A comprehensive guide for the recognition and classification of distinct stages of hair follicle morphogenesis. J Invest Dermatol. 1999;113:523–532
  21. Muller-Rover S, Handjiski B, Van DV, Eichmuller S, Foitzik K, McKay IA, et al. A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages. J Invest Dermatol. 2001;117:3–15
  22. Lindner G, Menrad A, Gherardi E, Merlino G, Welker P, Handjiski B, et al. Involvement of hepatocyte growth factor/scatter factor and met receptor signaling in hair follicle morphogenesis and cycling. FASEB J. 2000;14:319–332
  23. Ohnemus U, Unalan M, Handjiski B, Paus R. Topical estrogen accelerates hair regrowth in mice after chemotherapy-induced alopecia by favoring the dystrophic catagen response pathway to damage. J Invest Dermatol. 2004;122:7–13
  24. Schneider MR, Schmidt-Ullrich R, Paus R. The hair follicle as a dynamic miniorgan. Curr Biol. 2009;19:R132–R142
  25. Klein J, Permana PA, Owecki M, Chaldakov GN, Bohm M, Hausman G, et al. What are subcutaneous adipocytes really good for?. Exp Dermatol. 2007;16:45–70
  26. Fajas L. Adipogenesis: a cross-talk between cell proliferation and cell differentiation. Ann Med. 2003;35:79–85
  27. Harrington M, Pond-Tor S, Boney CM. Role of epidermal growth factor and ErbB2 receptors in 3T3-L1 adipogenesis. Obesity (Silver Spring). 2007;15:563–571
  28. Serrero G, Mills D. Physiological role of epidermal growth factor on adipose tissue development in vivo. Proc Natl Acad Sci USA. 1991;88:3912–3916
  29. Serrero G. EGF inhibits the differentiation of adipocyte precursors in primary cultures. Biochem Biophys Res Commun. 1987;146:194–202
  30. Kurachi H, Adachi H, Ohtsuka S, Morishige K, Amemiya K, Keno Y, et al. Involvement of epidermal growth factor in inducing obesity in ovariectomized mice. Am J Physiol. 1993;265:E323–E331
  31. Adachi H, Kurachi H, Homma H, Adachi K, Imai T, Morishige K, et al. Epidermal growth factor promotes adipogenesis of 3T3-L1 cell in vitro. Endocrinology. 1994;135:1824–1830
  32. Boney CM, Smith RM, Gruppuso PA. Modulation of insulin-like growth factor I mitogenic signaling in 3T3-L1 preadipocyte differentiation. Endocrinology. 1998;139:1638–1644
  33. Lu Z, Hasse S, Bodo E, Rose C, Funk W, Paus R. Towards the development of a simplified long-term organ culture method for human scalp skin and its appendages under serum-free conditions. Exp Dermatol. 2007;16:37–44
  34. Ciardiello F, Tortora G. EGFR antagonists in cancer treatment. N Engl J Med. 2008;358:1160–1174
  35. Schneider MR, Antsiferova M, Feldmeyer L, Dahlhoff M, Bugnon P, Hasse S, et al. Betacellulin regulates hair follicle development and hair cycle induction and enhances angiogenesis in wounded skin. J Invest Dermatol. 2008;128:1256–1265
  36. Mak KK, Chan SY. Epidermal growth factor as a biologic switch in hair growth cycle. J Biol Chem. 2003;278:26120–26126
  37. Dahlhoff M, Muller AK, Wolf E, Werner S, Schneider MR. Epigen transgenic mice develop enlarged sebaceous glands. J Invest Dermatol. 2009;
  38. Hollis DE, Chapman RE. Apoptosis in wool follicles during mouse epidermal growth factor (mEGF)-induced catagen regression. J Invest Dermatol. 1987;88:455–458
  39. Chapman RE, Hardy MH. Effects of intradermally injected and topically applied mouse epidermal growth factor on wool growth, skin and wool follicles of merino sheep. Aust J Biol Sci. 1988;41:261–268
  40. Philpott MP, Kealey T. Effects of EGF on the morphology and patterns of DNA synthesis in isolated human hair follicles. J Invest Dermatol. 1994;102:186–191
  41. Lacouture ME. Mechanisms of cutaneous toxicities to EGFR inhibitors. Nat Rev Cancer. 2006;6:803–812

PII: S0923-1811(09)00363-6

doi: 10.1016/j.jdermsci.2009.12.004

« Back Journal of Dermatological Science

Article in Press