Age-related changes of p75 Neurotrophin receptor-positive adipose-derived stem cells

Yamada, Takaaki; Akamatsu, Hirohiko; Hasegawa, Seiji; Yamamoto, Naoki; Yoshimura, Tomohisa; Hasebe, Yuichi; Inoue, Yu; Mizutani, Hiroshi; Uzawa, Tohru; Matsunaga, Kayoko; Nakata, Satoru

doi:10.1016/j.jdermsci.2010.02.003

« Previous Next »Journal of Dermatological Science
Volume 58, Issue 1 , Pages 36-42, April 2010

Age-related changes of p75 Neurotrophin receptor-positive adipose-derived stem cells

Takaaki Yamada
- Research Laboratories, Nippon Menard Cosmetic Co., Ltd., 2-7 Torimicho, Nishi-Ku, Nagoya, Aichi 451-0071, Japan
- Department of Dermatology, Fujita Health University School of Medicine, 1-98 Kutsukakecho, Toyoake, Aichi 470-1192, Japan
- Corresponding author at: Research Laboratories, Nippon Menard Cosmetic Co., Ltd., 2-7 Torimicho, Nishi-Ku, Nagoya, Aichi 451-0071, Japan. Tel.: +81 52 531 6263; fax: +81 52 531 6277.
Hirohiko Akamatsu
- Department of Applied Cell and Regenerative Medicine, Fujita Health University School of Medicine, 1-98 Kutsukakecho, Toyoake, Aichi 470-1192, Japan
Seiji Hasegawa
- Research Laboratories, Nippon Menard Cosmetic Co., Ltd., 2-7 Torimicho, Nishi-Ku, Nagoya, Aichi 451-0071, Japan
- Department of Dermatology, Fujita Health University School of Medicine, 1-98 Kutsukakecho, Toyoake, Aichi 470-1192, Japan
Naoki Yamamoto
- Laboratory of Molecular Biology & Histochemistry, Fujita Health University Joint Research Laboratory, 1-98 Kutsukakecho, Toyoake, Aichi 470-1192, Japan
Tomohisa Yoshimura
- Research Laboratories, Nippon Menard Cosmetic Co., Ltd., 2-7 Torimicho, Nishi-Ku, Nagoya, Aichi 451-0071, Japan
Yuichi Hasebe
- Research Laboratories, Nippon Menard Cosmetic Co., Ltd., 2-7 Torimicho, Nishi-Ku, Nagoya, Aichi 451-0071, Japan
Yu Inoue
- Research Laboratories, Nippon Menard Cosmetic Co., Ltd., 2-7 Torimicho, Nishi-Ku, Nagoya, Aichi 451-0071, Japan
Hiroshi Mizutani
- Research Laboratories, Nippon Menard Cosmetic Co., Ltd., 2-7 Torimicho, Nishi-Ku, Nagoya, Aichi 451-0071, Japan
Tohru Uzawa
- Graduate School of Mathematics, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan
Kayoko Matsunaga
- Department of Dermatology, Fujita Health University School of Medicine, 1-98 Kutsukakecho, Toyoake, Aichi 470-1192, Japan
Satoru Nakata
- Research Laboratories, Nippon Menard Cosmetic Co., Ltd., 2-7 Torimicho, Nishi-Ku, Nagoya, Aichi 451-0071, Japan

Received 13 July 2009; received in revised form 18 January 2010; accepted 7 February 2010. published online 12 July 2010.

Abstract

Background

The existence of multipotent stem cells in subcutaneous adipose tissue has been reported. We previously confirmed that p75 neurotrophin receptor (p75NTR; CD271)-positive cells in subcutaneous adipose tissue possessed multipotency, although changes of the characteristics in p75NTR-positive adipose-derived stem cells (ASCs) with aging remain unclear.

Objective

To investigate the effect of aging on p75NTR-positive ASCs.

Methods

The number of p75NTR-positive ASCs in subcutaneous adipose tissue of ICR mice aged 3–24 weeks was analyzed by immunostaining and flow cytometry. Subsequently, the cells were isolated and their ability to attach to the cell culture dish, proliferation rate (doubling time) and the expression of senescence-associated β-galactosidase (SA-β gal), a cellular senescence marker, were assessed. Age-related changes in the differentiation potential of p75NTR-positive cells in adipogenic, osteogenic, chondrogenic and myogenic lineage were also investigated.

Results

The number of ASCs per unit of tissue weight in adipose tissue and the attachment rate of isolated cells decreased with aging. No difference in the cell proliferation rate and the percentage of SA-β gal-positive cells was detected. Although the efficacy of differentiation into adipogenic and osteogenic lineages slightly decreased with aging, the differentiation potential into chondrogenic and myogenic lineages was not changed.

Conclusion

The number of ASCs per unit of tissue weight decreased in aged mice. However, the cells possessed proliferation and differentiation potentials almost equal to those of young mice even though the differentiation potentials showed a tendency of decrease. These results raise the possibility that stem cell functions, self-renewal and multipotency, are maintained regardless of aging.

Keywords: p75 neurotrophin receptor (p75NTR, CD271), Adipose-derived stem cells (ASCs), Aging