Journal of Dermatological Science
Volume 59, Issue 1 , Pages 60-62 , July 2010

A protocol for enrichment of CD34+ stromal cell fraction through human skin disaggregation and magnetic separation

  • Araika Gutiérrez-Rivera

      Affiliations

    • Rare Disease Program, Skin Regeneration Laboratory, Fundación Inbiomed, San Sebastián, Spain
    • Regenerative Medicine Program, Instituto Biodonostia, Hospital Donostia, San Sebastián, Spain
  • Ander Izeta

      Affiliations

    • Rare Disease Program, Skin Regeneration Laboratory, Fundación Inbiomed, San Sebastián, Spain
    • Regenerative Medicine Program, Instituto Biodonostia, Hospital Donostia, San Sebastián, Spain
    • Corresponding Author InformationCorresponding author at: Instituto Biodonostia, Hospital Donostia, Paseo Doctor Beguiristain, 115, 20014 San Sebastián, Spain. Tel.: +34 94 3006249; fax: +34 94 3006250.

Received 8 January 2010

References 

  1. Poblet E, Jimenez F, Godinez JM, Pascual-Martin A, Izeta A. The immunohistochemical expression of CD34 in human hair follicles: a comparative study with the bulge marker CK15. Clin Exp Dermatol. 2006;31:807–812
  2. Erdag G, Qureshi HS, Patterson JW, Wick MR. CD34-positive dendritic cells disappear from scars but are increased in pericicatricial tissue. J Cutan Pathol. 2008;35:752–756
  3. Nickoloff BJ. The human progenitor cell antigen (CD34) is localized on endothelial cells, dermal dendritic cells, and perifollicular cells in formalin-fixed normal skin, and on proliferating endothelial cells and stromal spindle-shaped cells in Kaposi's sarcoma. Arch Dermatol. 1991;127:523–529
  4. Kirchmann TT, Prieto VG, Smoller BR. CD34 staining pattern distinguishes basal cell carcinoma from trichoepithelioma. Arch Dermatol. 1994;130:589–592
  5. Tardio JC. CD34-reactive tumors of the skin. An updated review of an ever-growing list of lesions. J Cutan Pathol. 2009;36:89–102
  6. Tellechea O, Reis JP, Ilheu O, Baptista AP. Dermal cylindroma. An immunohistochemical study of thirteen cases. Am J Dermatopathol. 1995;17:260–265
  7. Aiba S, Tabata N, Ohtani H, Tagami H. CD34+ spindle-shaped cells selectively disappear from the skin lesion of scleroderma. Arch Dermatol. 1994;130:593–597
  8. Gutiérrez-Rivera A, Pavón-Rodríguez A, Jiménez-Acosta F, Poblet E, Braun KM, Cormenzana P, et al. Functional characterization of highly adherent CD34 positive keratinocytes isolated from human skin. Exp Dermatol;. 2010;in press
  9. Arts CH, de Groot P, Heijnen-Snyder GJ, Blankensteijn JD, Eikelboom BC, Slaper-Cortenbach IC. Application of a clinical grade CD34-mediated method for the enrichment of microvascular endothelial cells from fat tissue. Cytotherapy. 2004;6:30–42
  10. Trempus CS, Morris RJ, Bortner CD, Cotsarelis G, Faircloth RS, Reece JM, et al. Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34. J Invest Dermatol. 2003;120:501–511

PII: S0923-1811(10)00132-5

doi: 10.1016/j.jdermsci.2010.04.011

Journal of Dermatological Science
Volume 59, Issue 1 , Pages 60-62 , July 2010

Article Tools

* Image Usage & Resolution