Role of plasminogen activator inhibitor-2 (PAI-2) in keratinocyte differentiation

Jang, Sunhyae; Yang, Tae Ho; An, Eun Jeong; Yoon, Hyun Kyung; Sohn, Kyung-Cheol; Cho, Ah Young; Ryu, Eun-Kyoung; Park, Yeon-suk; Yoon, Tae Young; Lee, Jeung-Hoon; Kim, Chang Deok

doi:10.1016/j.jdermsci.2010.04.012

« Previous Next »Journal of Dermatological Science
Volume 59, Issue 1 , Pages 25-30, July 2010

Role of plasminogen activator inhibitor-2 (PAI-2) in keratinocyte differentiation

Sunhyae Jang
- Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, 55 Munhwa-ro, Daejeon 301-747, Republic of Korea
- These authors contributed equally to this study.
Tae Ho Yang
- Department of Dermatology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea
- These authors contributed equally to this study.
Eun Jeong An
- Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, 55 Munhwa-ro, Daejeon 301-747, Republic of Korea
Hyun Kyung Yoon
- Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, 55 Munhwa-ro, Daejeon 301-747, Republic of Korea
Kyung-Cheol Sohn
- Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, 55 Munhwa-ro, Daejeon 301-747, Republic of Korea
Ah Young Cho
- Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, 55 Munhwa-ro, Daejeon 301-747, Republic of Korea
Eun-Kyoung Ryu
- Department of Nursing, Kongju National University, Kongju, Republic of Korea
Yeon-suk Park
- Department of Nursing, Kongju National University, Kongju, Republic of Korea
Tae Young Yoon
- Department of Dermatology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea
Jeung-Hoon Lee
- Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, 55 Munhwa-ro, Daejeon 301-747, Republic of Korea
Chang Deok Kim
- Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, 55 Munhwa-ro, Daejeon 301-747, Republic of Korea
- Corresponding author. Tel.: +82 42 580 8346, fax: +82 42 581 9475.

Received 30 October 2009; received in revised form 1 April 2010; accepted 23 April 2010. published online 12 July 2010.

Abstract

Background

Plasminogen activator inhibitor-2 (PAI-2) is an enzyme inhibitor which is involved in various biological processes including cell differentiation, tissue regrowth and regeneration. Although PAI-2 has been originally isolated as an extracellular inhibitor of urokinase plasminogen activator (uPA), recent studies indicate that PAI-2 has other intracellular effects in keratinocyte, such as the component of cornified envelope.

Objective

The aim of this study is to investigate the expression and functional role of PAI-2 during the keratinocyte differentiation.

Methods

We transduced keratinocytes with adenovirus harboring the expression cassette for PAI-2, then examined the effect on keratinocytes differentiation.

Results

When cultured epidermal keratinocytes were treated with 1.2mM calcium, PAI-2 expression was increased time-dependently at both mRNA and protein levels. The calcium-induced PAI-2 expression was abolished by treatment with p38 MAPK inhibitor, while overexpression of MKK6 led to the increase of PAI-2 expression. When PAI-2 was overexpressed by adenoviral transduction, the expression of keratinocyte differentiation markers such as involucrin, keratin 10 and loricrin was markedly increased. Concomitantly, overexpression of PAI-2 resulted in the retardation of cell growth, with the increase of Rb and p53.