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Regular paper| Volume 2, ISSUE 5, P353-360, September 1991

The effect of 1,25-dihydroxyvitamin D3 on the growth and differentiation of cultured human outer root sheath cells from normal subjects and patients with vitamin D-dependent rickets type II with alopecia

DOI:https://doi.org/10.1016/0923-1811(91)90029-W
The effect of 1,25-dihydroxyvitamin D3 on the growth and differentiation of cultured human outer root sheath cells from normal subjects and patients with vitamin D-dependent rickets type II with alopecia
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      Abstract

      We examined the effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on the growth and differentiation of cultured human hair outer root sheath cells (ORSC) from normal subjects and patients with vitamin D-dependent rickets type II (DDR-II) with alopecia. 1,25(OH)2D3 dose-dependently suppressed the plating efficiency, clonal growth, and DNA synthesis of normal ORSC. It enhanced the cornified envelope formation and caused morphological changes in the cells. All results indicated the existence of specific receptors for 1,25(OH)2D3 in the ORSC, and suggest that 1,25(OH)2D3 is a potent inhibitor of proliferation of ORSC as well as a stimulator of terminal differentiation. However, the cells from DDR-II patients with alopecia did not respond to 1,25(OH)2D3, suggesting a lack of the specific receptors in the cells. The differences in the cellular response to the hormone between the normal ORSC and those from the patients were apparent and easily distinguishable, therefore this experiment may be a rapid and simple diagnostic test for DDR-II patients with alopecia.
      Large number of hairs were difficult to obtain from patients with alopecia, and we developed a new culture method to accomplish these studies from a few plucked hair follicles. Our system may be useful in the culture of ORSC from limited number of follicles, and could be utilized to analyse the cellular characteristics of ORSC in patients with hair diseases.

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      References

        • Franceski RT
        • Simpson RU
        • DeLuca HF
        Binding proteins for vitamin D metabolite in serum carriers and intracellular receptors.
        Arch Biochem Biophys. 1981; 210: 1-13
        • DeLuca HF
        • Schnoes HK
        Vitamin D: recent advances.
        Annu Rev Biochem. 1983; 52: 411-439
        • Smith EL
        • Walworth NC
        • Holick MF
        Effect of 1α,25-dihydroxyvitamin D3 on the morphologic and biochemical differentiation of cultured human epidermal keratinocytes grown in serum-free conditions.
        J Invest Dermatol. 1986; 86: 709-714
        • Hosomi J
        • Hosoi J
        • Abe E
        • Suda T
        • Kuroki T
        Regulation of terminal differentiation of cultured mouse epidermal cells by 1α,25-di-hydroxyvitamin D3.
        Endocrinology. 1983; 113: 1950-1957
        • Stumpf WE
        • Sar M
        • Reid FA
        • Tanaka Y
        • DeLuca HF
        Target cells for 1,25-dihydroxyvitamin D3 in intestinal tract, stomach, kidney, skin, pituitary, and parathyroid.
        Science. 1976; 206: 1188-1190
        • Feldman D
        • Chen T
        • Hirst M
        • Colston K
        • Karasek M
        • Cone C
        Demonstration of 1,25-dihydroxyvitamin D3 receptors in human skin biopsies.
        J Clin Endcrinol Metabol. 1980; 51: 1463-1465
        • Rosen JF
        • Fleishman AR
        • Finberg L
        • Hamstra A
        • DeLuca HF
        Rickets with alopecia: an inborn error of vitamin D metabolism.
        J Pediat. 1979; 94: 729-735
        • Sockalosky JJ
        • Ulstrom RA
        • DeLuca HF
        • Brown DM
        Vitamin D-resistant rickets: Endorgan unresponsiveness to 1,25(OH)2D3.
        J Pediat. 1980; 96: 701-703
        • Liberman UA
        • Eil C
        • Marx J
        Clinical features of hereditary resistance to 1,25-dihydroxyvitamin D3 (hereditary hypocalcemic vitamin D resistant rickets type II).
        Adv Exp Med Biol. 1984; 78: 391-406
        • Kuwana R
        • Arase S
        • Sadamoto Y
        • Nakanishi H
        • Takeda K
        A method for culturing human hair follicle cells(II).
        Nishinihon J Dermatol. 1988; 50 (in Japanese): 271-276
        • Tanaka H
        • Abe E
        • Miyaura C
        • Kuribayashi T
        • Konno K
        • Nishii Y
        • Suda T
        1α,25-dihydroxycholecalciferol and a human myeloid leukemia cell line (HL-60). The presence of a cytosol receptor and induction of differentiation.
        Biochem J. 1982; 204: 713-719
        • Sun T-T
        • Green H
        Differentiation of the epidermal keratinocyte in cell culture: formation of the cornified envelope.
        Cell. 1976; 9: 511-521
        • Takeda E
        • Kuroda Y
        • Saijo T
        • Naito E
        • Kobashi H
        • Yokota I
        • Miyao M
        1α-hydroxyvitamin D3 treatment of three patients with 1,25-dihydroxyvitamin D-receptor-defect rickets and alopecia.
        Pediatrics. 1987; 80: 97-101
        • Arase S
        • Sadamoto Y
        • Kuwana R
        • Watanabe Y
        • Nakanishi H
        • Shigemi F
        • Takeda K
        • Takeda E
        Five cases of vitamin D-dependent rickets type II with alopecia.
        Jpn J dermatol. 1988; 98 (in Japanese): 1029-1038
        • Amento EP
        • Bhalla AK
        • Kurnick JT
        • Kradin RL
        • Clemens TL
        • Holick SA
        • Holick MF
        • Krane SM
        1α,25-dihydroxyvitamin D3 induces maturation of human monocytes cell line U937, and in association with a factor from human T lymphocytes, augments production of the monokine, mononuclear cell factor.
        J Clin Invest. 1984; 73: 731-739
        • Clemens TL
        • Adams JS
        • Horiuchi N
        • Gilchrest BA
        • Cho H
        • Tsuchiya Y
        • Matsuo N
        • Suda T
        • Holick MF
        Interaction of 1,25-dihydroxyvitamin D3 with keratinocytes and fibroblasts from skin of normal subjects and a subject with vitamin D-dependent rickets type II: a model for study of the mode of action of 1,25-dihydroxyvitamin D3.
        J Clin Endcrinol Metabol. 1983; 56: 824-830
        • Lieberman UA
        • Eil C
        • Holst P
        • Rosen JF
        • Marx SJ
        Hereditary resistance to 1,25-dihydroxyvitamin D3: defective function of receptor for 1,25-dihydroxyvitamin D3 in cells cultured from bone.
        J Clin Endocrinol Metab. 1983; 57: 958-962
        • Takeda E
        • Kuroda Y
        • Saijo T
        • Toshima K
        • Naito E
        • Kobashi H
        • Iwakuni Y
        • Miyao M
        Rapid diagnosis of vitamin D-dependent rickets type II by use of phytohemagglutinin-stimulated lymphocytes.
        Clinica Chimica Acta. 1986; 155: 245-250
        • Fujita T
        • Nomura M
        • Okajima S
        • Furuya H
        Adult-onset vitamin D-resistant osteomalacia with the unresponsiveness to parathyroid hormone.
        J Clin Endocrinol Metab. 1980; 50: 927-931
        • Weterings PJJM
        • Vermorken AJM
        • Bloemendal H
        A method for culturing hair follicle cells.
        Br J Dermatol. 1981; 104: 1-5
        • Limat A
        • Noser FK
        Serial cultivation of single keratinocytes from the outer root sheath of human scalp hair follicles.
        J Invest Dermatol. 1986; 87: 485-488

      Article info

      Publication history

      Accepted: April 22, 1991
      Received: August 11, 1990

      Identification

      DOI: https://doi.org/10.1016/0923-1811(91)90029-W

      Copyright

      © 1991 Published by Elsevier Inc.

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