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Abstract
The study describes the vertical distribution of free amino acids in the porcine epidermis
as compared to the human integument, using a micro-determination TLC method based
on the reaction of amino acids with dansyl chloride. This microanalytical approach
demonstrated 22 free amino acids, with the relatively largest amounts being present
for acidic amino acids and their amides. It was obvious that the relative amounts
of certain amino acids (alanine, proline, valine, glutamine, histidine, glycine, threonine)
decreased, whereas acidic amino acids (glutamic acid, aspartic acid) increased from
the stratum basale up to the stratum corneum. This distributional pattern could be
verified for the dorsal and lateral body regions of the pig breeds used, and for man.
The results obtained are discussed in view of the development of epidermal keratinization.
Keywords
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References
- 2nd ed. Biochemistry and Physiology of the Skin. Vol. 1. Oxford Univ Press, New York, Oxford1983
- Keratohyalin.in: Bereiter-Hahn J Matoltsy AG Richards KS 2nd ed. Biology of the Integument, Vol. 2, Vertebrates. Springer, Berlin, Heidelberg, New York, Tokyo1986: 739-751
- 2nd ed. Die Haut des Schweines (The Porcine Integument). Schlütersche, Hannover1986
- Pools and protein synthesis in mammalian cells.Biochem J. 1979; 178: 699-709
- Freie Aminosäuren und Harnstoff in psoriatischen und normalen, epidermalen Verhornungsprodukten.Arch Klin Exp Derm. 1966; 225: 299-305
- Freie Aminosäuren in Ichthyosis-Schuppen und in Callus.Arch Klin Exp Derm. 1969; 235: 43-52
- Studies on the biochemistry of epidermis. IV. The free amino acids, ammonia, urea, and pyrrolidone carboxylic acid content of conventional and germ-free albino guinea pig epidermis.J Invest Dermatol. 1970; 54: 24-31
- Zur Veränderung der relativen Konzentrationen der freien Aminosäuren im Stratum corneum menschlicher Epidermis durch gezielte Umwelteinflüsse. I. Der Einfluß von UV-Strahlung.Arch Derm Res. 1977; 259: 157-160
- Micromethods in Molecular Biology.in: 2nd ed. Molecular Biology, Biochemistry and Biophysics. Vol. 14. Springer, Berlin, Heidelberg, New York1973
- Thin layer chromatography of dansyl and dinitrophenyl derivatives of amino acids. A review.Biomed Chromatogr. 1989; 3: 233-240
- Selected micromethods for use in neurochemistry.in: Lajtha A 2nd ed. Handbook of Neurochemistry. Vol. 2. Plenum Press, New York1982: 349-396
- Arbeitsmethoden der makroskopischen und mikroskopischen Anatomie.Fischer, Stuttgart1964
- Comparative aspects of free amino acids in the central nervous system of spiders.Comp Biochem Physiol. 1980; 67C: 83-86
- Automatische Fluorometrie von Mikrodünnschichtchromatogrammen.Fres Z Analyt Chem. 1978; 290: 133
- Quantitative Auswertung von zweidimensionalen Mikro-Dünnschichtchromatogrammen.in: Nagel HH Digitale Bildverarbeitung Informatik Fachberichte. Vol. 8. Springer, Berlin1977: 12-20
- The epidermal cell periphery.in: Goldsmith LA Biolchemistry and Physiology of the Skin. Vol. 1. Oxford University Press, New York, Oxford1986: 184-196
- Epidermal keratins.in: Goldsmith LA Biochemistry and Physiology of the Skin. Vol. 1. Oxford University Press, New York, Oxford1986: 135-169
- The proteins of keratohyalin.in: Goldsmith LA Biochemistry and Physiology of the Skin. Vol. 1. Oxford University Press, New York, Oxford1986: 170-183
- Filaggrin: a keratin filament associated protein.Ann NY Acad Sci. 1985; 455: 330-342
- Identification of two intermediates during processing of profilaggrin to filaggrin in neonatal mouse epidermis.J Cell Biol. 1984; 99: 1372-1378
- Identification of proteolytic cleavage sites in the conversion of profilaggrin to filaggrin in mammalian epidermis.J Biol Chem. 1989; 264: 1837-1845
- Differentially localized incorporation of amino acids in relation to epidermal keratinization in the newborn rat.Anat Rec. 1965; 152: 525-536
- Ultrastructural autoradiographic studies of keratohyalin granule formation.J Invest Dermatol. 1967; 49: 595-604
- A comparative autoradiographic study of keratohyalin granules containing cystine and histidine.J Ultrastruct Res. 1975; 51: 314-325
- Changing patterns of incorporation of [14C] histidine and [3H] leucine into epidermal proteins during differentiation of fetal rat skin.J Invest Dermatol. 1975; 65: 482-487
- Biochemistry.Harper and Row, New York1983
- Structure of glycoproteins and their oligosaccharide units.in: Lennarz WJ The Biochemistry of Glycoproteins and Proteoglycans. Plenum Press, New York, London1980: 1-34
- Histochemistry of complex carbohydrates in the hairy skin of the domestic pig.Histochem J. 1983; 15: 845-860
- Lectins as markers of human epidermal cell differentiation.Differentiation. 1982; 22: 205-210
- Alterations in membrane sugars during epidermal differentiation: visualization with lectins and role of glycosidases.J Histochem Cytochem. 1983; 31: 887-897
- Distribution of glycoconjugates in normal human skin using biotinyl lectins and avidin-horseradish peroxidase.Histochemistry. 1983; 79: 1-7
- Distribution of carbohydrate residues in normal skin.Arch Dermatol Res. 1984; 276: 216-223
- Lysosomes and the skin.J Invest Dermatol. 1975; 65: 259-271
- Lysosomal hydrolases of the epidermis.Br J Dermatol. 1975; 92: 391-398
- The distribution of enzymes in the skin of the domestic pig.Lab Animals. 1976; 10: 237-247
- The epidermis.in: Jadassohn J Handbuch der Haut- und Geschlechtskrankheiten, Ergw I/1. Springer, Berlin, Heidelberg, New York1968: 1-142
- Biochemie der Haut. Thieme, Stuttgart1973
- Structure and function of epidermal proteases and their inhibitors.in: Ogawa H The Biological Role of Proteinases and their inhibitors in Skin. Elsevier, Amsterdam1986: 39-48
- Amino acid catabolism.in: Miflin BJ The Biochemistry of Plants. Vol. 5. Academic Press, New York1980: 541-567
- Role of protein and calcium in stratum corneum cell cohesion.Arch Dermatol Res. 1987; 279: 184-189
- Natural moisturizing factors in skin.Am Perfum Cosm. 1967; 82: 77-79
- Pyrrolidone carboxylic acid synthesis in guinea pig epidermis.J Invest Dermatol. 1983; 81: 122-124
- Histidine-rich proteins (Filaggrins): structural and functional heterogeneity during epidermal differentiation.J Mol Biol. 1983; 170: 651-673
Article info
Publication history
Accepted:
May 27,
1991
Received:
February 12,
1991
Identification
Copyright
© 1991 Published by Elsevier Inc.
