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Abstract
Mouse epidermal keratinocyte-derived Pam 212 cells were irradiated with UV light,
and the culture media were examined for plasminogen activator (PA) activity by measuring
the capacity to convert exogenous plasminogen into plasmin. Exposure of cells to a
broad spectrum of light in the UVB range induced a significant elevation of PA activity
at 16 h after irradiation. A dose-response study revealed that a maximal enhancement,
15-fold higher than non-irradiated controls, was induced at a sublethal UVB dose of
100 J/m2, which significantly inhibited cell proliferation without affecting cell viability.
Addition of 5 μg/ml of cycloheximide lowered the UV-induced elevation of PA activity,
suggesting that protein synthesis is required for this phenomenon. Action spectra
for PA synthesis were obtained by irradiating cells with monochromatic light ranging
from 250 to 360 mm, and the data demonstrated that the action spectrum was 250–320
nm in length with a peak between 260 and 280 nm. The results suggest that UV exposure
is an important physiological trigger for modulating PA synthesis in the epidermis.
Keywords
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Article info
Publication history
Accepted:
March 16,
1992
Received:
March 15,
1990
Identification
Copyright
© 1992 Published by Elsevier Inc.
