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Abstract
This study was attempted to produce the monoclonal antibodies specific for Treponema pallidum and to investigate their characteristics, thereby contributing to identify the antigenic
structure and to apply the diagnosis of syphilis. The seven clones (YS 3, YS 75, YS
307, YS 481, YS 343, YS 1 and YS 406) secreting monoclonal antibodies reactive with
T. pallidum were produced. The isotypes of the seven monoclonal antibodies produced were defined.
Optical densities of the 7 monoclonal antibodies were ranged from 0.59 to 1.48 as
measured by ELISA. Monclonal antibodies from 5 of the 7 clones which could agglutinate
sheep RBC sensitized with T. pallidum, showed strong fluorescences. The monoclonal antibody from YS 343 was cross-reactive
with non-pathogenic treponemes, but the other 6 monoclonal antibodies reacted with
T. pallidum specifically. On immunoblotting, monoclonal antibodies from 5 of the 7 clones reacted
with a polypeptide of a molecular weight of 47 kDa, and monoclonal antibodies from
YS 343 reacted with a polypeptide of 64 kDa common to both T. pallidum and T. phagedenis. The above results revealed that 7 clones secreting monoclonal antibodies reactive
to T. pallidum were produced successfully, and specific antibodies reacting with major antigenic
polypeptides of a molecular weight of 47 kDa would be useful in the diagnosis of syphilis
in the future.
Keywords
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Article info
Publication history
Accepted:
December 27,
1991
Received:
December 13,
1990
Identification
Copyright
© 1992 Published by Elsevier Inc.
