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Abstract
Decrease in CD4+CD45RA+ lymphocytes in peripheral blood of systemic lupus erythematosus (SLE) patients has
been reported. In this study, mononuclear cells were separated from peripheral blood
of SLE patients, and the CD4+CD45RA+ cells were counted by flow cytometry just after separation and also 1 week after
incubation in vitro. Furthermore, healthy lymphocytes were incubated with SLE patient's
sera, and the Ca2+ level was measured to investigate the interaction of patient's sera with healthy
lymphocytes. The CD4+CD45RA+ lymphocytes were found to be decreased in number on the first day just after separation
of lymphocytes, but recovered to normal levels after 1 week culture without patient's
sera. The intracellular Ca2+ level in normal lymphocytes increased 1 min after incubation with patient's sera,
but not with healthy control sera. These results suggest that CD4+CD45RA+ cells are persistently activated in the peripheral blood of SLE patients, and that
their sera contain some extrinsic factors which could activate the lymphocytes.
Keywords
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References
- Differences in the surface proteins of mouse B and T cells.in: Proc Natl Acad Sci USA. 72. 1975: 157-161
- The leukocyte common antigen (CD45): a putative receptor-linked protein tyrosine phosphatase.in: Proc Natl Acad Sci USA. 85. 1988: 7182-7186
- CD45 regulates signal transduction and lymphocyte activation by specific association with receptor molecules on T or B cells.in: Proc Natl Acad Sci USA. 85. 1988: 8628-8632
- A defect of immunoregulatory T cell subsets in systemic lupus erythematosus patients demonstrated with anti-2H4 antibody.J Clin Invest. 1987; 79: 762-768
- Quantitative defect of CD4+2H4+ cells in systemic lupus erythematosus and Sjogren's syndrome.Arthritis Rheum. 1987; 30: 1407-1411
- Complete exon-intron organization of the human leukocyte common antigen (CD45) gene.J Immunol. 1988; 15: 2781-2787
- Demonstration that the leukocyte common antigen CD45 is a protein tyrosine phosphatase.Biochemistry. 1988; 27: 8695-8701
- Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens.J Exp Med. 1987; 166: 1548-1566
- Cyclic regulation of CD45 isoform expression in a long term human CD4+CD45RA+ T cell line.J Immunol. 1991; 146: 1175-1183
- Reversal of CD45 isoform switching in CD8+ T cells.Clin Immunol. 1993; 25: 107-114
- Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator.J Cell Biol. 1982; 94: 325-334
- Free cytoplasmic calcium concentration and the mitogenic stimulation of lymphocytes.J Biol Chem. 1983; 258: 4876-4882
- The role of the 2H4 molecule in the generation of suppressor function in con A-activated T cells.J Immunol. 1986; 137: 3247-3253
- Loss of activation-induced CD45RO+ with maintenance of CD45RA+ expression during prolonged culture of T cells and NK cells.Immunology. 1991; 74: 78-85
- IgG anti-lymphocyte antibodies in systemic lupus erythematosus react with surface molecules shared by peripheral T cells and a primitive T cell line.J Immunol. 1987; 138: 1750-1756
- Identification of three major target molecules of IgM antilymphocyte autoantibodies in systemic lupus erythematosus.J Immunol. 1987; 139: 3644-3651
- Antilymphocyte antibodies against CD4+2H4+ cell populations in patients with systemic lupus erythematosus.Arthritis Rheum. 1989; 32: 398-405
- Autoantibodies specific for different isoforms of CD45 in systemic lupus erythematosus.J Exp Med. 1990; 172: 653-656
- Soluble interleukin-2 receptors in systemic lupus erythematosus.Arthritis Rheum. 1988; 31: 729-735
- Soluble interleukin-2 receptor levels in serum and its relationship to T cell abnormality and clinical manifestations of the disease in patients with systemic lupus erythematosus.J Rheum. 1991; 18: 831-836
- The pathogenesis of SLE and IL-10.Clin Immunol. 1993; 25: 43-51
Article info
Publication history
Accepted:
December 27,
1993
Received in revised form:
October 29,
1993
Received:
June 28,
1993
Identification
Copyright
© 1994 Published by Elsevier Inc.
