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Abstract
The cyclic growth activity of the hair follicle is characterized by substantial remodelling
of the extracellular matrix, yet, little is known about the proteolytic activities
regulating this process. In murine skin, hair cycling is highly synchronized and is
associated with dramatic remodelling of all skin compartments. We therefore have assessed,
in this pilot study, proteolytic activities of murine skin from various stages of
the depilation-induced hair cycle. We show that the defined proteolytic activities
displayed by organ cultured intact mouse skin differ between hair cycle stages. Skin
with all follicles in telogen or mid anagen displayed only minimal lysis of collagen
type I gels, while early anagen skin had significant collagenase activity. Skin cultured
on gelatin gels at the air-liquid interphase (‘histoculture’) completely lysed the
gel within 5 days when all follicles were in early anagen, while this was not observed
with mid and very lateanagen skin. Zymography of conditioned medium from these cultures
revealed the secretion of activated interstitial collagenase and of gelatinases of
72 and 92 kDa, with the maximum of interstitial collagenase activity secreted by anagen
IV skin. Addition of TPA or TNF-α to the culture medium stimulated secreted collagenase
type I activity. The C 57 BL-6 mouse offers an attractive model for dissecting and
manipulating hair cycle-associated proteolysis in a physiologically relevant system.
Keywords
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Article info
Publication history
Accepted:
February 15,
1994
Received in revised form:
December 3,
1993
Received:
July 8,
1993
Identification
Copyright
© 1994 Published by Elsevier Inc.
