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Abstract
The effects of Werner's syndrome (WS) fibroblast-conditioned medium on cell proliferation
and collagen production of normal fibroblasts were studied using four WS fibroblast
strains. The conditioned medium from the WS fibroblasts brought about activation of
normal fibroblast proliferation, whereas, that from late passage normal fibroblasts
and fibroblasts from aged donors' skin did not. Collagen and non-collagenous protein
synthesis in normal fibroblasts were increased by the addition of conditioned medium
from the WS fibroblasts, but the relative rates of collagen synthesis and non-collagenous
protein synthesis were unaltered. These results suggest that activation of fibroblast
proliferation by conditioned medium is one of the characteristics of WS fibroblasts.
Keywords
Abbreviations:
DMEM (Dulbecco's modified Eagle's medium), FBS (fetal bovine serum), PBS (phosphate-buffered saline), PDL (population doubling level), WS (Werner's syndrome)To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
March 3,
1994
Received in revised form:
January 10,
1994
Received:
October 20,
1993
Identification
Copyright
© 1994 Published by Elsevier Inc.
