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Abstract
A simple method is described for the procurement of human blood eosinophil phenotypes
by combining an anti-CD16 monoclonal antibody, immunobeads, and a non-toxic and non-ionic
density gradient medium, Nycodenz. The purification depends on the removal of mononuclear
cells using a 1.076/1.102 g/ml Nycodenz density gradient, partial removal of neutrophils
based on different binding to plastic dishes, interaction of residual neutrophils
with immunobeads via an anti-CD16 monoclonal antibody and, finally, extraction of
eosinophil phenotypes by sifting the immunobeads-loaded neutrophils through an 1.080/1.102
g/ml Nycodenz density gradient. This method permits simultaneous preparation of highly
purified normodense (>1.080 g/ml) and hypodense eosinophils (<1.080 g/ml) with reasonable
chemiluminescence responses to opsonized zymosans and helminthotoxic activity to opsonized
schistosomula corresponding to their own immunocytological properties.
Keywords
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receptor expression on normal density and low density human eosinophils.Article info
Publication history
Accepted:
December 19,
1994
Received in revised form:
November 28,
1994
Received:
June 30,
1994
Identification
Copyright
© 1995 Published by Elsevier Inc.
