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Abstract
The alteration of main disaccharide units in the skin of hairless mice (HOS:Hr 1)
after chronic and repeated ultraviolet light (UV) radiation was investigated using
high performance liquid chromatography after labeling with 1-phenyl-3-methyl-5-pyrazolone.
The total amount of main disaccharide units increased by UVA irradiation at the 24th
and 36th weeks in comparison with the control. At the 36th week UVA significantly
increased hyaluronic acid-derived ΔDi-HA (HA). Dermatan sulfate-derived ΔDi 4S (DS)
and chondroitin sulfate-derived ΔDi-4S (CS) increased at the 36th week, although not
statistically significant. The total amount of main disaccharide units was increased
significantly by UYB irradiation at the 24th week as compared with the control. Concerning
the compositional change in main disaccharide units after a 36-week repeated exposure,
the decrease in ΔDi-HA(HA) and the increase in ΔDi-4S (DS) were found in the order
of control, UVA- and UVB-irradiated groups. These results, for the first time, indicate
the precise alterations of glycosaminoglycans, both in the total amount and in the
composition, confirming the previous histochemical findings. This disaccharide analysis
should provide a useful method to examine the biochemical changes of skin glycosaminoglycans
in photoaging.
Keywords
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References
- The hairless mouse and photoaging.Photochem Photobiol. 1991; 54: 1109-1118
- The histochemistry of chronically sun-damaged skin. An investigation of mucopolysaccharides and basophilia in actinically damaged skin using alcian blue, Mowry's and Hicks-Mattahaei stains, methylation, and saponification.J Invest Dermatol. 1961; 37: 447-452
- Hexosamine and hydroxyproline alterations in chronically sun-damaged skin.in: 3rd ed. Proc Soc Exp Biol Med. 108. 1961: 533-535
- Alterations in human dermal connective tissue with age and chronic sun damage.J Invest Dermatol. 1962; 139: 347-350
- An attempt to produce elastosis in aged human skin by means of ultraviolet radiation.J Invest Dermatol. 1968; 50: 225-226
- The ultraviolet-irradiated hairless mouse: a model for photoaging.J Am Acad Dermatol. 1989; 21: 623-631
- An animal model of solar-aged skin: histological, physical, and visible changes in UV-irradiated hairless mouse skin.Photochem Photobiol. 1987; 46: 367-378
- Wavelength dependence of histological, physical, and visible changes in chronically UV-irradiated hairless mouse skin.Photochem Photobiol. 1989; 50: 763-769
- Chronic ultraviolet B radiation-induced biochemical changes in the skin of hairless mice.Photochem Photobiol. 1990; 51: 91-97
- Connective tissue alterations in the skin of ultraviolet irradiated hairless mice.J Invest Dermatol. 1988; 91: 158-161
- The effect of high and low ultraviolet-B dose exposure on the degree of hairless mouse skin wrinkling.Photochem Photobiol. 1991; 53: 109-112
- Biochemical changes in hairless mouse skin collagen after chronic exposure to ultraviolet- A radiation.Photochem Photobiol. 1991; 54: 233-237
- Effects on chronic exposure ultraviolet-A including 2% ultraviolet-B on free radical reduction systems in hairless mice.Photochem Photobiol. 1991; 54: 737-740
- UVA-induced ultrastructural changes in hairless mouse skin: a comparison to UVB- induced damage.J Invest Dermatol. 1993; 100: 194-199
- High-performance liquid chromatography of reducing carbohydrates as strongly ultraviolet- absorbing and electrochemically sensitive 1-phenyl-3-methyl-5-pyrazolone derivatives.Anal Biochem. 1989; 180: 351-357
- Disaccharide analysis of the skin glycosaminoglycans in systemic sclerosis.Br J Dermatol. 1992; 126: 29-34
- Changes in skin disaccharide components correlate with the severity of sclerotic skin in systemic sclerosis.Acta Derm Venereol (Stockh). 1994; 74: 179-182
- Alterations of proteoglycans in ultraviolet-irradiated skin.Photochem Photobiol. 1993; 58: 211-218
Article info
Publication history
Accepted:
December 28,
1994
Received in revised form:
December 16,
1994
Received:
August 11,
1994
Identification
Copyright
© 1995 Published by Elsevier Inc.
