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The alteration of main disaccharide units in the skin of hairless mice (HOS:Hr 1) after chronic and repeated ultraviolet light (UV) radiation was investigated using high performance liquid chromatography after labeling with 1-phenyl-3-methyl-5-pyrazolone. The total amount of main disaccharide units increased by UVA irradiation at the 24th and 36th weeks in comparison with the control. At the 36th week UVA significantly increased hyaluronic acid-derived ΔDi-HA (HA). Dermatan sulfate-derived ΔDi 4S (DS) and chondroitin sulfate-derived ΔDi-4S (CS) increased at the 36th week, although not statistically significant. The total amount of main disaccharide units was increased significantly by UYB irradiation at the 24th week as compared with the control. Concerning the compositional change in main disaccharide units after a 36-week repeated exposure, the decrease in ΔDi-HA(HA) and the increase in ΔDi-4S (DS) were found in the order of control, UVA- and UVB-irradiated groups. These results, for the first time, indicate the precise alterations of glycosaminoglycans, both in the total amount and in the composition, confirming the previous histochemical findings. This disaccharide analysis should provide a useful method to examine the biochemical changes of skin glycosaminoglycans in photoaging.
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Accepted: December 28, 1994
Received in revised form: December 16, 1994
Received: August 11, 1994
© 1995 Published by Elsevier Inc.