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The regulation of leukocytes-endothelial cells binding by biological response modifiers have an important role in determining the progression of acute and chronic inflammatory responses. In order to define the influence of E-selectin on the binding of T lymphocytes to human dermal microvascular endothelial cells (HDMEC), we examined the cell surface expression of E-selectin on HDMEC and the regulation of the binding of T lymphocytes to HDMEC by IFN-γ. We have demonstrated that stimulation of HDMEC with IL-1α or TNFα leads to transient E-selectin induction which disappears after 48 h, but stimulation of HDMEC with IFN-γ resulted in delayed E-selectin induction which was seen at 48 h of incubation and persisted until 72 h after stimulation. However, stimulation with IFN-γ failed to induce E-selectin expression on human umbilical vein endothelial cells. The delayed E-selectin expression on HDMEC by IFN-γ coincided with the increases in T lymphocyte binding to IFN-γ-activated HDMEC. The binding of memory T lymphocytes to IFN-γ-activated HDMEC was greater than that of naive T lymphocytes. Anti-E-selectin antibody partially inhibited memory T lymphocyte binding to HDMEC after 48 h of stimulation with IFN-γ. These data show that E-selectin expressions by IFN-γ on endothelial cells are regulated in a tissue-specific fashion and that E-selectin may be important in vivo in the preferential migration of memory T lymphocytes into inflammatory sites in the skin.
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- Interlukin 1 acts on cultured human vascular endothelium to increase the adhesion of polymorphonuclear leukocytes, monocytes, and related cell lines.J Clin Invest. 1985; 76: 2003-2011
- Effects of bacterial lipopolysaccharide on the binding of lymphocytes to endothelial cell monolayers.J Immunol. 1986; 136: 569-573
- Adhesion molecules controlling lymphocyte migration.Cell. 1989; 56: 907-910
- Two distinct monokines, interleukin 1 and tumor necrosis factor, each independently induce biosynthesis and transient expression of the same antigen on the surface of cultured human vascular endothelial cells.J Immunol. 1986; 136: 1680-1687
- Identification of an inducible endothelial-leukocyte adhesion molecule.in: Proc Natl Acad Sci USA. 84. 1987: 9238-9242
- Endothelial leucocyte adhesion molecule-1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins.Science. 1989; 243: 1160-1165
- Cytokines and endothelial cell biology.Immunol Rev. 1990; 70: 427-451
- Endothelial leucocyte adhesion molecule-1 (ELAM-1) expression in cutaneous inflammation.Br J Dermatol. 1991; 124: 117-123
- T-cells bind to cytokine-activated endothelial cells via a novel, inducible sialoglycoprotein and ELAM-1.J Immunol. 1990; 145: 819-830
- Activation-independent binding of human memory T cells to adhesion molecule ELAM-1.Nature. 1991; 349: 799-802
- ELAM-1 is an adhesion molecule for skin-homing T cells.Nature. 1991; 349: 796-799
- Role of laminin and basement membrane in the morphological differentiation of human endothelial cells into capillary-like structures.J Cell Biol. 1988; 107: 1589-1598
- VCAM-1-, ELAM-1-, and ICAM-1-independent adhesion of melanoma cells to cutured human dermal microvascular endothelial cells.J Invest Dermatol. 1992; 98: 79-85
- Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria.J Clin Invest. 1973; 52: 2745-2756
- Immunomagnetic purification of T cell subpopulations.in: Coligan JE Kruisbeek AM Margulies DH Shevach EM Strober W Current Protocols in Immunology. John Wiley and Sons, New York1991: 7.4.1-7.4.6
- Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune in terferon.J Immunol. 1986; 137: 1893-1896
- Studies of the modulation of MHC antigen and cell adhesion molecule expression on human dermal microvascular endothelial cells.J Invest Dermatol. 1991; 97: 190-196
- IFN-γ enhances endothelial activation induced by tumor necrosis factor but not IL-1.J Immunol. 1990; 145: 1727-1733
- IFN gamma regulates the expression of the adhesion molecule ELAM-1 and IL-6 production by human endothelial cells in vitro.J Immunol. 1990; 145: 2110-2114
- Regulation of vascular cell adhesion molecule 1 on human dermal microvascular endothelial cells.J Immunol. 1992; 149: 698-705
- Human dermal microvascular endothelial but not human umbilical vein endothelial cells express CD36 in vivo and in vitro.J Immunol. 1992; 148: 78-83
- Human gamma Interferon increases the binding of T lymphocytes to endothelial cells.Clin Exp Immunol. 1985; 62: 554
- Human dermal microvascular endothelial cells behave like umbilical vein endothelial cells in T-cell adhesion studies.J Invest Dermatol. 1987; 88: 340-344
Accepted: April 11, 1995
Received in revised form: March 22, 1995
Received: October 22, 1994
© 1995 Published by Elsevier Inc.