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Abstract
The regulation of leukocytes-endothelial cells binding by biological response modifiers
have an important role in determining the progression of acute and chronic inflammatory
responses. In order to define the influence of E-selectin on the binding of T lymphocytes
to human dermal microvascular endothelial cells (HDMEC), we examined the cell surface
expression of E-selectin on HDMEC and the regulation of the binding of T lymphocytes
to HDMEC by IFN-γ. We have demonstrated that stimulation of HDMEC with IL-1α or TNFα
leads to transient E-selectin induction which disappears after 48 h, but stimulation
of HDMEC with IFN-γ resulted in delayed E-selectin induction which was seen at 48
h of incubation and persisted until 72 h after stimulation. However, stimulation with
IFN-γ failed to induce E-selectin expression on human umbilical vein endothelial cells.
The delayed E-selectin expression on HDMEC by IFN-γ coincided with the increases in
T lymphocyte binding to IFN-γ-activated HDMEC. The binding of memory T lymphocytes
to IFN-γ-activated HDMEC was greater than that of naive T lymphocytes. Anti-E-selectin
antibody partially inhibited memory T lymphocyte binding to HDMEC after 48 h of stimulation
with IFN-γ. These data show that E-selectin expressions by IFN-γ on endothelial cells
are regulated in a tissue-specific fashion and that E-selectin may be important in
vivo in the preferential migration of memory T lymphocytes into inflammatory sites
in the skin.
Keywords
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Article info
Publication history
Accepted:
April 11,
1995
Received in revised form:
March 22,
1995
Received:
October 22,
1994
Identification
Copyright
© 1995 Published by Elsevier Inc.
