Abstract
Skin equivalent model provides a new investigating system to study the role of extracellular
matrix and dermal factors such as collagen, basement membrane components and fibroblasts
(Fb) which contribute to cell-cell and cell-matrix interactions. Although basement
membrane factors is known to play an important role in epidermal differentiation and
epidermal-matrix adhesion, comparative effects of these extracellular matrix and dermal
factors on the reconstruction of epidermis are little known. In this study, we investigated
effects of type I collagen (Coll I), type IV collagen plus laminin (LAM) coated Coll
I (Coll IV+LAM), and human Fb enriched Coll I (Coll I+Fb) on epidermal reconstruction.
When human keratinocytes were cultured on three different gels containing Coll I,
Coll IV+LAM and Coll I+Fb, basal keratinocytes were cuboidal and perpendicular to
the dermo-epidermal junction only in the gel containing Coll I+Fb. Proliferation marker
expression was prominent and differentiation marker expression was similar with those
of normal skin in the gel containing Coll I+Fb than in the other gel models. Since
ascorbate is suspected to exert an effect as a modulator of proliferation and differentiation
in keratinocytes, we tested the effects of ascorbate on human epidermis reconstruction.
When 25 μg/ml ascorbate was added, disordered arrangement of epidermis was disappeared
and differentiation marker expression was similar with its expression in normal skin.
These data indicate that human Fb and a modulator of proliferation and differentiation
such as ascorbate are essential for epidermalization in reconstructed epidermis.
Keywords
Abbreviations:
Coll I, type I collagen (), Coll IV, type IV collagen (), LAM, laminin (), Fb, fibroblasts (), DED, de-epidermized dermis (), LSE, living skin equivalent ()To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
August 21,
2002
Received in revised form:
August 8,
2002
Received:
June 17,
2002
Identification
Copyright
© 2002 Elsevier Science Ireland Ltd. Published by Elsevier Inc. All rights reserved.
