Summary
Background:
Our previous study showed that T cells in skin lesions of human atopic dermatitis
(AD) had oligoclonal accumulation, indicating the involvement of antigen-specific
immune reactions at those sites. Recently, NC/Nga mice, which develop skin lesions
similar to AD, have been proposed as a model for that disease.
Objective:
To clarify whether NC/Nga mice are suitable as a model for human AD from the viewpoint
of their antigen-specific immune responses.
Methods:
Reverse transcription-polymerase chain reaction (RT-PCR) and single strand conformation
polymorphism (SSCP) analyses were conducted to detect TCR BV genes of clonally expanded
T cells derived from NC/Nga mice at an early phase of the AD-like dermatitis, at a
late phase of the dermatitis, and with no AD-like dermatitis.
Results:
(1) T cells with TCR BV 7, 10 and 17 reside in the skin of NC/Nga mice without the
AD-like dermatitis. (2) T cells with these BV genes contain oligoclonal accumulations,
however, expanded T cell clonotypes are also detected in the spleen and exist constantly
during the course of the AD-like dermatitis. (3) Development of the AD-like dermatitis
is associated with additional oligoclonal expansion/accumulation of T cells with TCR
BV 2, 4 and 6 genes. (4) Progression of the AD-like dermatitis is associated with
further oligoclonal expansion/accumulation of T cells with the TCR BV 14 gene. (5)
Some of the expanded TCR clonotypes are common between the individual mice and between
early and late phases.
Conclusions:
Taking these data together with the previous human AD studies, NC/Nga mice seem to
be an appropriate model for human AD.
Keywords
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Article info
Publication history
Accepted:
November 25,
2004
Received in revised form:
November 18,
2004
Received:
August 10,
2004
Identification
Copyright
© 2004 Japanese Society for Investigative Dermatology. Published by Elsevier Inc. All rights reserved.
