Summary
Background:
Acne vulgaris is a chronic inflammatory disease involving colonization of Propionibacterium acnes (P. acnes), activation of neutrophils and lymphocytes. Circumstantial evidence suggests that
antigen-independent and -dependent immune responses against P. acnes are involved in the pathogenesis of inflammatory acne. Epidermal keratinocytes are
also suggested to be involved in initiation and progression of cutaneous inflammation.
Nadifloxacin, a fluorinated quinolone, has potent antimicrobial activities against
Gram-negative and -positive microbes and is used to treat multiple inflamed acne lesions.
However, its effect on immune conferring cells such as mononuclear cells and keratinocytes
has not been examined.
Objective:
To evaluate the possible involvement of potential anti-inflammatory activity of nadifloxacin
in its therapeutic effect on inflammatory acne, we examined the effects of nadifloxacin,
in comparison with other antibiotics used to treat acne vulgaris, on cytokine production
by human peripheral blood mononuclear cells (PBMC) and keratinocytes.
Methods:
Cytokine production by PBMC was determined after treatment with heat-killed P. acnes in the presence or absence of antimicrobials using a real-time PCR and ELISA. Cultured
human epidermal keratinocytes were stimulated by IFN-γ plus IL-1β and the effects
of antimicrobials were examined by using ELISA.
Results:
Nadifloxacin as well as macrolide antibiotics and clindamycin inhibited IL-12 and
IFN-γ production by PBMC stimulated by heat-killed P. acnes. The drug also inhibited the IL-1α, Il-6, IL-8 and GM-CMS production by keratinocytes
treated with IFN-γ plus IL-1β.
Conclusions:
Inhibitory effects of nadifloxacin to activate T cells and keratinocytes may be involved
at least in part in the mechanism of its therapeutic effect against inflammatory acne.
Abbreviations:
PBMC (peripheral blood mononuclear cell), NDFX (nadifloxacin), LVFX (levofloxacin)Keywords
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Article info
Publication history
Accepted:
January 5,
2005
Received in revised form:
January 4,
2005
Received:
August 31,
2004
Identification
Copyright
© 2005 Japanese Society for Investigative Dermatology. Published by Elsevier Inc. All rights reserved.
