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Effect of liposome encapsulation of tea catechins on their accumulation in basal cell carcinomas

  • Jia-You Fang
    Correspondence
    Corresponding author at: Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 333, Taiwan. Tel.: +886 3 2118800x5521; fax: +886 3 2118236.
    Affiliations
    Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, Kweishan, Taoyuan, Taiwan
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  • Woan-Ruoh Lee
    Affiliations
    Department of Dermatology, Taipei Medical University Hospital, Taipei, Taiwan

    Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan
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  • Shing-Chuan Shen
    Affiliations
    Department of Dermatology, College of Medicine, Taipei Medical University, Taipei, Taiwan

    Department of Dermatology, Taipei Municipal Wan Fang Hospital, Taipei, Taiwan
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  • Yen-Ling Huang
    Affiliations
    Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, Kweishan, Taoyuan, Taiwan
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      Summary

      Background

      (−)-Epigallocatechin gallate (EGCG), the main active polyphenol in green tea, is associated with antioxidant and anticancer activities.

      Objective

      The aim of this study was to evaluate the feasibility of using liposomes for intratumor distribution of EGCG and its derivative, (+)-catechin.

      Method

      Liposomes containing egg phosphatidylcholine, cholesterol, or anionic surfactant in the presence of 15% ethanol were prepared. The physicochemical characteristics including vesicle size, zeta potential, drug entrapment, and drug release of liposomal formulations were determined. The liposomes containing EGCG were injected into basal cell carcinomas (BCCs), melanomas, and colon tumors to examine the tumor uptake of the drug. Liposomes were also incubated with a given number of BCC cells, and the cell viability was estimated.

      Result

      Almost no drug molecules were observed when free EGCG was administered to BCCs. EGCG encapsulated in liposomes with deoxycholic acid (DA) and ethanol increased drug deposition by 20-fold as compared to the free form. The larger vesicle size of this formulation was suggested to be the predominant factor governing this enhancement. The liposomes without ethanol showed low or negligible enhancement on EGCG uptake in BCCs. Liposomes protected EGCG from degradation, resulting in the induction of greater BCC death compared to that by free EGCG at lower concentrations.

      Conclusion

      These results suggest that the intratumor injection of liposomes containing EGCG with moderate modification is an effective approach for increasing EGCG deposition in BCCs.

      Keywords

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