Summary
Background
The recovery of RNA from the upper epidermis by tape stripping yields variable RNA
mass but has not been evaluated for its dependence on anatomical location. Gene expression
at different body locations and the origin of RNA recovered by tape stripping have
not been investigated.
Objectives
To characterize the recovery of RNA from different anatomical locations by tape stripping;
to correlate the recovery of RNA and removal of barrier by tape stripping, as assayed
by transepidermal water loss; and to investigate gene expression in the upper epidermis
at different body locations.
Methods
Twelve subjects were tape stripped at 15 body locations. RNA mass was evaluated and
gene expression assayed. Subjects were tape stripped 4, 8 and 12 times on the upper
back and transepidermal water loss and RNA recovery assayed.
Results
Ranked by median RNA recovery, the following order was observed: mastoid > forehead > chest > upper back > mid back > cheek > lower back > deltoid > forearm > abdomen > ventral thigh > inner arm > shin > dorsal thigh > lower leg. Expression of the housekeeping gene mRNAs is found to be uniform and reproducible
while IL-8 and TNFα mRNAs are expressed in different quantities both at different
body sites within an individual and between individuals at a specific anatomical site.
Data show a significant and high correlation between the number of tapes used to strip
a site and transepidermal water loss but no strong correlation between transepidermal
water loss and RNA recovery or number of tapes used to strip a site and RNA recovery.
Conclusions
Subjects and anatomical location are shown to be significantly different for the ability
to recover RNA by tape stripping. We hypothesize that RNA recovered by tape strip
is not derived from corneocytes but from cells associated with the stratum corneum.
Keywords
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Article info
Publication history
Accepted:
August 14,
2006
Received in revised form:
August 4,
2006
Received:
May 18,
2006
Identification
Copyright
© 2006 Japanese Society for Investigative Dermatology. Published by Elsevier Inc. All rights reserved.
