Abstract
Background
Benign and malignant fibrous histiocytoma present with a considerable difference concerning
cellular organization in their vicinity.
Objective
Normally appearing epithelium covers the malignant form in contrast to hyperplastic
epidermis for benign tumors. It is an open question as to whether the tumor-associated
fibroblasts are capable to affect phenotypic features of normal keratinocytes, prompting
this comparative analysis.
Methods
Fibroblasts were isolated from benign and malignant fibrous histiocytomas, respectively,
and also from normal dermis. The resulting cell populations were thoroughly characterized
immunocytochemically using a large panel of antibodies. The three fibroblast preparations
were cocultured with normal interfollicular keratinocytes. Their phenotype was characterized
for distinct properties including differentiation and proliferation.
Results
Fibroblasts prepared from both tumor types were phenotypically practically identical
with normal dermal fibroblasts. Their activities on keratinocytes were different.
Cells prepared from benign fibrous histiocytoma were capable to effect strong expression
of keratin 19 and production of a galectin-1-rich extracellular matrix. Fibroblasts
isolated from malignant fibrous histiocytoma led to a phenotype very similar to that
when keratinocytes were cocultured with normal dermal fibroblasts.
Conclusion
Fibroblasts prepared from benign fibrous histiocytoma were biologically active on
keratinocytes in a particular manner. Our results on fibroblast activity are suggested
to be relevant for morphologic differences observed in vivo between normal epidermis and epidermis adjacent to the studied tumor types.
Keywords
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Article info
Publication history
Published online: July 12, 2010
Accepted:
March 18,
2009
Received in revised form:
March 16,
2009
Received:
January 6,
2009
Identification
Copyright
© 2009 Japanese Society for Investigative Dermatology. Published by Elsevier Inc. All rights reserved.
