Abstract
Background
Sunscreens have long been used to protect against the acute effects of UV radiation.
They can also have protective effects on chronic UV-induced changes, such as photoaging
and skin cancer. Recent studies have focused on marine organisms as a source of natural
bioactive molecules and some UV-absorbing algal compounds are under investigation
as candidates for new natural sunscreens.
Objective
The cutaneous photoprotective ability of the mycosporine-like aminoacids (MAAs) Porphyra-334
and shinorine (P-334 + SH), high UV-absorbing compounds isolated from the red alga Porphyra rosengurttii, was evaluated by in vivo procedures in mouse skin. The expression of the heat shock protein HSP70 as a potential
biomarker for acute UV damage was also investigated.
Methods
A galenic formulation containing the MAA combination of P-334 + SH was applied topically to the dorsal skin of SkhR-1 H hairless mice, which were
irradiated with a single UV radiation dose of 3.87 J cm−2 and compared with a combination of UVB- and UVA-absorbing reference filters. Clinical
signs of sunburn, such as erythema and edema, as well as other quantifiable histological
and biochemical parameters, such as the expression of the heat shock protein 70 and
antioxidant enzyme activities, were measured from skin biopsies at 6, 24, 48 and 72 h post-radiation.
Results
The formulation containing MAA prevented sunburn cell formation, as well as corneum
stratum, malphigian, dermal and hypodermal thickening and other structural and morphological
alterations observed in biopsies of non-photoprotected skin. A significant increase
in Hsp70 was observed in the epidermis of non-photoprotected mouse skin, besides a
de novo expression in deeper layers. P-334 + SH protected against the significant decrease in superoxide dismutase and catalase
activities observed in non-photoprotected mice.
Conclusion
The topical application of P-334 + SH protected against UV-induced skin damage in mice and contributed to maintaining
the antioxidant defence system of the skin as well as Hsp70 expression.
Keywords
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Article info
Publication history
Published online: July 12, 2010
Accepted:
June 2,
2009
Received in revised form:
May 20,
2009
Received:
September 9,
2008
Identification
Copyright
© 2009 Japanese Society for Investigative Dermatology. Published by Elsevier Inc. All rights reserved.
