Abstract
Background
Tissue glycation, the main cause of many diabetes-related complications, results in
the accumulation of advanced glycation endproducts (AGE).
Objectives
These AGEs are endogenous fluorophores that can serve as a viable pathological indicator
for disease diagnostics. Here we explore the capabilities of multiphoton microscopy
to non-invasively localize and quantify the skin glycation.
Methods
In our study, multiphoton microscopy and spectroscopy were used to investigate glycation
events-induced changes in the intensities of autofluorescence and second harmonic
generation on ex vivo human skin.
Results
Temporal and spatial dependence of degrees of glycation of the epidermis, collagen
and elastin fibers of dermis were evaluated for their relevance to the changes in
amplitudes of autofluorescence signals. We found that glycation drastically and linearly
increases multiphoton autofluorescence intensity of epidermis and dermal collagen
whereas changes in dermal elastin are moderate. We also found decrease in the level
of second harmonic generation signal.
Conclusion
Our study suggests that due to intrinsically weak autofluorescence the dermal collagen
is the most sensitive skin tissue to be used for detecting changes in tissue glycation.
Abbreviations:
AGE (advanced glycation endproducts), MPAF (multiphoton autofluorescence), SHG (second harmonic generation)Keywords
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Article info
Publication history
Published online: January 25, 2012
Accepted:
December 10,
2011
Received in revised form:
November 20,
2011
Received:
August 3,
2011
Identification
Copyright
© 2012 Japanese Society for Investigative Dermatology. Published by Elsevier Inc. All rights reserved.
