Abstract
Background
Dry skin causes pruritus and discomfort in patients with xerosis and atopic dermatitis.
General treatment for skin dryness involves the topical application of an emollient.
However, more effective, simpler therapies are desired. Collagen tripeptide (CTP)
is a highly purified, non-antigenic, low-allergenic collagen fraction that is known
to have various biological effects.
Objective
To clarify the therapeutic effects of CTP for dry skin using acetone-induced dry skin
model mice.
Methods
ICR mice were treated with acetone followed by oral administration of CTP (80 or 500 mg/kg/day) for 3 days. Hyaluronic acid production induced by CTP was assessed using
human dermal fibroblasts in vitro and in an acetone-induced dry skin model mice in
vivo. Transepidermal water loss (TEWL) and scratching behavior were evaluated. Furthermore,
the effects of CTP on intraepidermal nerve fibers and expression of semaphorin 3A
(Sema3A) and nerve growth factor (NGF) were examined by immunohistochemistry and quantitative
RT-PCR.
Results
CTP enhanced hyaluronic acid production in human dermal fibroblasts in vitro and in
murine skin in vivo. Oral administration of CTP in acetone-induced dry skin model
mice significantly decreased TEWL and suppressed scratching behavior. Intraepidermal
nerve growth was dramatically inhibited in CTP-treated mice. Quantitative PCR analysis
and immunohistochemical study revealed that CTP abolished the increased NGF and decreased
Sema3A levels induced by acetone treatment.
Conclusion
Oral administration of CTP improves dry skin and normalizes axon-guidance factors
in the epidermis in addition to reducing pruritus. CTP may be used in a new therapeutic
strategy against dry skin and pruritus.
Keywords
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Article info
Publication history
Published online: March 14, 2012
Accepted:
February 8,
2012
Received in revised form:
February 5,
2012
Received:
December 8,
2011
Identification
Copyright
© 2012 Japanese Society for Investigative Dermatology. Published by Elsevier Inc. All rights reserved.
