Abstract
Background
The use of non-thermal atmospheric pressure plasma in dermatology to improve the healing
of chronic wounds is a promising application. The antimicrobial properties of physical
plasma offer on the one hand the killing of bacteria, which are often a problem in
chronic wounds. On the other hand, plasma can activate cells which are involved in
the wound closure.
Objective
To guarantee a safe application it is essential to understand basic interactions between
physical plasma and human skin cells.
Methods
In our study, human keratinocytes (HaCaT cells) were directly plasma treated with
a dielectric barrier discharge (DBD) plasma source and effects on viability, DNA,
cell cycle, intracellular concentration of reactive oxygen species and induction of
apoptosis were observed.
Results
A treatment time-dependent loss of recovered adherent HaCaT cells after 24 h and a linear increase of DNA damage were observed, which was no longer evident 24 h after plasma stimulation, except for long treatment times. An accumulation of HaCaT
cells in G2/M phase and a decrease in the G1 phase was caused by DBD plasma. The increasing
formation of intracellular ROS is also attributed to plasma treatment. In contrast
to other studies we did not find clear evidences for apoptosis in adherent HaCaT cells.
A culture medium exchange subsequently after plasma treatment weakened the observed
effects.
Conclusion
DBD plasma treatment resulted in oxidative stress in human keratinocytes which is
related to deficient cell performance.
Keywords
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Article info
Publication history
Published online: April 24, 2013
Accepted:
January 31,
2013
Received in revised form:
January 28,
2013
Received:
August 14,
2012
Identification
Copyright
© 2013 Japanese Society for Investigative Dermatology. Published by Elsevier Inc. All rights reserved.
