Abstract
Background
Barrier-to-autointegration factor 1 (BANF1) is an essential component of the nuclear
lamina. Recent studies have clarified that BANF1 is a causative molecule of Nestor-Guillermo
progeria syndrome. Despite recent progress in studies on BANF1, the role of BANF1
in keratinocytes has not been addressed at all.
Objective
This study aims to determine the localization of BANF1 in psoriatic epidermal keratinocytes
as well as in normal keratinocytes and to clarify its possible function in those keratinocytes.
Methods
Immunohistochemistry of BANF1 was performed on 10 cases of psoriasis and 10 healthy
control individuals. Expression of molecules associated with inflammation of the skin
by HSC-1, a human skin squamous cell carcinoma cell line, stimulated by TPA and treated
with siRNA to BANF1 were analyzed with quantitative PCR and Western blot.
Results
Strong nuclear-dominant immunostaining of BANF1 was seen in the epidermal keratinocytes
of psoriatic lesions, although in the normal epidermis, all the KCs in the upper epidermis
showed cytoplasmic-dominant staining of BANF1. By BANF1 knockdown in TPA-stimulated
HSC-1 cells, the mRNA levels of S100A9 were significantly elevated compared with those
of control HSC-1 cells treated with siRNA to CD4. The protein expression level of
S100A9 and phosphorylated c-Jun was elevated by BANF1 knockdown.
Conclusion
BANF1 is translocated onto the nuclear envelope in the psoriatic epidermal keratinocytes,
suggesting that BANF1 is associated with upregulated proliferation of keratinocytes
in psoriatic lesions. Activation of BANF1 possibly suppresses S100A9 expression and
inactivates c-Jun, resulting in suppression of cutaneous inflammation.
Keywords
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Article info
Publication history
Published online: May 10, 2013
Accepted:
April 14,
2013
Received in revised form:
March 29,
2013
Received:
November 29,
2012
Identification
Copyright
© 2013 Japanese Society for Investigative Dermatology. Published by Elsevier Inc. All rights reserved.
