Abstract
Background
Pigmentation is controlled by complex mechanisms. Evidence suggests that miRNAs can
regulate pigmentation. However, the mechanism has not been fully elucidated. Objective
In this study, we revealed a novel mechanism that regulates pigmentation involving
exosomes, miRNAs and the crosstalk between keratinocytes and melanocytes.
Methods
The expression and localization of exosome specific marker TSG101 in keratinocytes
and melanocytes; Changes of melanin content in melanocytes after co-culture of exosome
and melanocytes; Expression changes of target gene TYR and its related genes and inhibitory
effect of miR-330-5p on pigmentation were studied by using various molecular biological
techniques.
Results
In this experiment, we used miR-330-5p in keratinocytes to verify the effect of keratinocyte
derived exosome on melanocyte pigmentation. First, we found that keratinocytes secrete
exosomes carrying miR-330-5p; moreover, greater miR-330-5p expression was found in
exosomes derived from keratinocytes that overexpressed miR-330-5p. Second, we found
that exosomes derived from keratinocytes with overexpression of miR-330-5p caused
a significant increase in miR-330-5p in melanocytes. Finally, exosomes derived from
keratinocytes that overexpressed miR-330-5p induced a significant decrease in the
production of melanin and expression of TYR in melanocytes. Meanwhile, we overexpressed
miR-330-5p in melanocytes, which also proved the inhibitory effect of miR-330-5p on
pigmentation.
Conclusion
These findings suggest that keratinocytes crosstalk with melanocytes in the epidermal
melanin unit via exosomal miRNAs. These studies reveal an important role of exosomes
in melanocyte pigmentation, which opens a new pathway of melanogenesis.
Keywords
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Article Info
Publication History
Published online: March 12, 2019
Accepted:
February 12,
2019
Received in revised form:
January 28,
2019
Received:
July 13,
2018
Identification
Copyright
© 2019 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.
