Highlights
- •Pretreatment of visible red light diminished the UV-induced damage in NHDFs.
- •Transcriptomic analysis of red light-exposed NHDFs revealed UV-protective pathway.
- •HSPA1A, HSPA5, PTGS2, IL6, LIF, and ATF3 had a key role in the protective pathway.
Abstract
Background
Ultraviolet B (UVB) radiation is a major cause of skin photodamage, including the
damage associated with photodermatoses, aging, and cancer. Although many studies have
shown that red light has photoprotective effects on skin, the mechanisms underlying
these effects are still poorly understood.
Objective
The aim of this study was to identify the photoprotective effects of visible red light
against UVB-induced skin damage in normal human dermal fibroblast cells using a transcriptomic
approach.
Methods
Next-generation sequencing-based transcriptomic analyses were used to profile transcriptomic
alterations and identify genes that are differentially expressed by visible red light
and by UVB exposure. To understand the biological networks among identified genes,
a literature-based biological pathway analysis was performed. Quantitative real-time
polymerase chain reaction assays were used for mRNA-level validation of selected key
genes.
Results
We observed that visible red light contributes to skin cell protection against UVB
by modulating gene expression that enhances the adaptive response to redox and inflammatory
balancing and by upregulating genes involved in DNA excision repair processes. We
also identified that several key genes in the red light-induced biological network
were differentially regulated.
Conclusions
Visible red light enhanced the UVB-protective effects in normal human skin cells via the transcriptomic modulation of genes involved in cell-protective processes. Our
findings from this next-generation sequencing analysis may lead to a better understanding
of the cytoprotective effects of visible red light and provide direction for further
molecular or mechanistic studies.
Keywords
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Article info
Publication history
Published online: March 23, 2019
Accepted:
March 20,
2019
Received in revised form:
March 19,
2019
Received:
July 23,
2018
Identification
Copyright
© 2019 Published by Elsevier B.V. on behalf of Japanese Society for Investigative Dermatology.
