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DNA methylation markers in peripheral blood for psoriatic arthritis

  • Min Deng
    Affiliations
    Department of Dermatology, The Second Xiangya Hospital, Central South University, Changsha, China
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  • Yuwen Su
    Affiliations
    Department of Dermatology, The Second Xiangya Hospital, Central South University, Changsha, China
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  • Ruifang Wu
    Affiliations
    Department of Dermatology, The Second Xiangya Hospital, Central South University, Changsha, China
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  • Siying Li
    Affiliations
    Department of Dermatology, The Second Xiangya Hospital, Central South University, Changsha, China
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  • Yanshan Zhu
    Affiliations
    Department of Dermatology, The Second Xiangya Hospital, Central South University, Changsha, China
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  • Guishao Tang
    Affiliations
    Department of Dermatology, The Second Xiangya Hospital, Central South University, Changsha, China
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  • Xiaoli Shi
    Affiliations
    Department of Dermatology, The Second Xiangya Hospital, Central South University, Changsha, China
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  • Tian Zhou
    Affiliations
    Department of Dermatology, The Second Xiangya Hospital, Central South University, Changsha, China
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  • Ming Zhao
    Correspondence
    Correspondence to: Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital, Central South University, 139 Renmin Middle Road, Changsha, Hunan 410011, China.
    Affiliations
    Department of Dermatology, The Second Xiangya Hospital, Central South University, Changsha, China
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  • Qianjin Lu
    Correspondence
    Correspondence to: Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Xuanwu Lake Street, Nanjing, Jiangsu, 210042, China.
    Affiliations
    Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China

    Key Laboratory of Basic and Translational Research on Immune-Mediated Skin Diseases, Chinese Academy of Medical Sciences, Nanjing, China

    Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Nanjing, China

    Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital, Central South University, Changsha, China
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Published:November 09, 2022DOI:https://doi.org/10.1016/j.jdermsci.2022.11.001

      Highlights

      • DNA methylation markers distinguish psoriatic arthritis from psoriasis vulgaris.
      • DNA methylation plays a crucial role in psoriasis and psoriatic arthritis.
      • DNA methylation serves as a good biomarker for the diagnosis of psoriatic arthritis.

      Abstract

      Background

      The clinical manifestations of psoriatic arthritis (PsA) are highly heterogeneous and no reliable diagnostic biomarkers exist.

      Objective

      We explored the role of DNA methylation CpG markers in the diagnosis of PsA.

      Methods

      DNA methylation array was used to screen for differentially methylated sites (DMSs) in the discovery phase (PsA, n = 25; healthy controls [HCs], n = 19; psoriasis vulgaris [PsV], n = 20). In the validation phase, pyrosequencing was used to identify the DMSs in an expanded cohort (PsA, n = 60; HCs, n = 91; PsV, n = 48; rheumatoid arthritis [RA], n = 60). Logistic regression prediction models were established based on the identified DMSs for the diagnosis of PsA.

      Results

      A total of 17 DMSs differentiating PsA and HCs as well as 11 DMSs differentiating PsA and PsV were screened in the discovery phase. A total of six DMSs (chr14: cg07940072, chr14: 38061320, chr9: cg15734589, chr6: cg12800266, chr3: cg12992827, chr6: cg24500972) differentiating PsA and HCs and two DMSs (chr12: cg16459382, chr2: cg16348668) differentiating PsA and PsV were identified using pyrosequencing. Three logistic regression prediction models were established based on the identified DMSs, which distinguished PsA, RA, PsV, and HCs (P < 0.001). The models performed well in differentiating PsA from HCs, RA, and PsV (AUC: 0.858, 0.851, and 0.976, respectively).

      Conclusions

      The models based on methylated CpG sites are useful for distinguishing patients with PsA from HCs and those with RA or PsV and are a highly sensitive and specific diagnostic biomarker for PsA.

      Abbreviations:

      PsA (psoriatic arthritis), PsV (psoriasis vulgaris), HCs (healthy controls), RA (rheumatoid arthritis), LASSO (least absolute shrinkage and selector operation), DMSs (differentially methylated sites), PBMCs (peripheral blood mononuclear cells)

      Keywords

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