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Abstract
The water content of the skin is greatly influenced by ground substances, which may
be responsible for wrinkling and laxity of the skin accompanying the cutaneous aging.
Therefore, water content in the skin is presumed to be a critical determinant in cutaneous
aging. This study was aimed at clarifying the change in water content and the content
of glycosaminoglycans (GAG) of rat skin in relation to aging. Sprague-Dawley rats
were divided into 6 groups: 1-, 3-, 6-, 12-, 18- and 24-month-old groups. Two-to-three
grams of skin tissue samples were taken from the back, and a half of sample was dried
at 160 ° C for 30 min with electronic moisture balance, and water content was assessed
as decreased weight by heating. To measure change of GAG of the rat skin, another
half of samples were extracted with 0.1 M sodium phosphate buffer (pH 7.4 NaPB) and
2 M guanidine-HCl/Tris buffer (pH 7.4). The resultant insoluble pellet was dried at
50 ° C in a drying oven for 72 h after two washings and the dry weight was recorded.
The amount of sulfated GAG in the skin extracts was measured by alcian blue precipitation
assay, and the amount of uronic acid (UA) was assayed in the skin tissue extracts
and the dried skin using the carbazole reaction. The water content of the rat skin
decreased with age, and a similar decreasing pattern in the amount of sulfated GAG
and UA of the rat skin tissue was observed with aging. One hundred times of UA was
obtained in dry rat skin tissue, as compared with that of the skin extracts. In conclusion,
there occurs a significant decrease of water content in the aged rat skin, which may
be related to the change of GAG with intrinsic aging of the skin.
Keywords
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Article info
Publication history
Accepted:
March 19,
1996
Received in revised form:
March 1,
1996
Received:
November 20,
1995
Identification
Copyright
© 1997 Published by Elsevier Inc.
