This paper is only available as a PDF. To read, Please Download here.
Abstract
Calponin (h1 isoform) was characterized as a smooth muscle specific, actin-, tropomyosin-,
calmodulin-binding protein and described as a factor which inhibits contraction. H2-calponin,
encoded by a different gene from h1-calponin, was identified from the smooth muscles
of mouse and pig. However, non-muscle calponin analogues have recently been reported
in rat and pig brains. Here we show the presence of calponin expressed in human skin
tissue and in cultured human keratinocytes using polyclonal antibodies to bovine aortic
smooth muscle calponin. Western blot analysis demonstrated that calponin with a molecular
weight around 36000 existed in extracts of keratinocytes. Immunofluorescence microscopy
displayed the localization of calponin in the cytoplasm of the basal cells in situ,
and along the cell-to-cell borders in cultured human keratinocytes maintained in standard
calcium medium. Furthermore, according to RT-PCR analysis using human h1- and h2-calponin-specific
primers, calponin expressed by cultured human keratinocytes was identified as the
h2 isoform. We demonstrated the presence of h2-calponin in human keratinocytes, and
it might play a role in the structural organization of actin cytoskeleton at the cytoplasmic
region of cell-to-cell junctions of keratinocytes.
Keywords
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Journal of Dermatological ScienceAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Isolation and characterization of a 34 000-dalton calmodulin- and F-actin-binding protein from chicken gizzard smooth muscle.Biochem Biophys Res Commun. 1986; 141: 20-26
- Vascular smooth muscle calponin. A novel troponin T-like protein.Hypertension. 1988; 11: 620-626
- Smooth muscle calponin.J Biol Chem. 1990; 265: 10148-10155
- Do thin filaments of smooth muscle contain calponin? A new method for the preparation.FEES Lett. 1990; 268: 165-168
- Effect of calponin on actin-activated myosin ATPase activity.J Biochem. 1990; 108: 835-838
- The mechanism for the inhibition of actin-activated ATPase of smooth muscle heavy meromyosin by calponin.Biochem Biophys Res Commun. 1991; 176: 1487-1493
- Functional interrelationship between calponin and caldesmon.Biochem J. 1991; 280: 33-38
- Inhibition of the relative movement of actin and myosin by caldesmon and calponin.J Biol Chem. 1992; 267: 15886-15892
- Occurrence of anti-gizzard P34K antibody cross-reactive components in bovine smooth muscles and non-smooth muscle tissues.Life Sci. 1987; 41: 291-296
- Cloning and expression of a novel acidic calponin isoform from rat aortic vascular smooth muscle.J Biol Chem. 1994; 269: 10683-10690
- Expression of an acidic isoform of calponin in rat brain. Western blots on one- or two-dimensional gels and immunolocalization in cultured cells.Biochem J. 1995; 306: 211-215
- Mammalian calponin. Identification and expression of genetic variants.FEBS Lett. 1993; 330: 13-18
- Molecular cloning and sequence analysis of smooth muscle calponin.J Biol Chem. 1991; 266: 13284-13288
- Human vascular smooth muscle calponin and SM22 share homology with myc and vav proto-oncogene products: structural characterization and functional implications.Circulation. 1991; 84: II-532
- Co-localization of immunoreactive forms of calponin with actin cytoskeleton in platelets, fibroblasts and vascular smooth muscle.J Biochem. 1991; 109: 311-316
- Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture.J Invest Dermatol. 1983; 81: 33s-40s
- Induction of polarized cell-cell association and retardation of growth by activation of the E-cadherin-catenin adhesion system in a dispersed carcinoma line.J Cell Biol. 1994; 127: 247-256
- Region-specific initiation of mouse mammary tumor virus RNA synthesis by endogenous RNA polymerase II in preparations of cell nuclei.J Biol Chem. 1983; 258: 2802-2807
- Changes of expression of intermediate filament proteins during ontogenesis of eccrine sweat glands.J Invest Dermatol. 1992; 98: 777-785
- Calcium-induced assembly of adherens junctions in keratinocytes.J Cell Biol. 1987; 105: 807-817
- The relationship between intermediate filaments and microfilaments before and during the formation of desmosomes and adherens-type junctions in mouse epidermal keratinocytes.J Cell Biol. 1987; 104: 1389-1402
- An immunofluorescence study of the calcium-induced co-ordinated reorganization of microfilaments, keratin intermediate filaments and microtubules in cultured human epidermal keratinocytes.J Invest Dermatol. 1991; 97: 985-994
- Regulation of keratinocyte intercellular junction organization and epidermal morphogenesis by E-cadherin.J Cell Biol. 1992; 117: 415-425
- Calcium regulation of growth and differentiation of mouse epidermal cells in culture.Cell. 1980; 19: 245-254
- Calcium regulation of cell- cell contact and differentiation of epidermal cells in culture. An ultrastructural study.Exp Cell Res. 1983; 143: 127-142
- Calcium-induced re-organization of desmosomal components in cultured human keratinocytes.J Cell Biol. 1984; 99: 2211-2215
Article info
Publication history
Accepted:
April 2,
1996
Received in revised form:
March 1,
1996
Received:
December 22,
1995
Identification
Copyright
© 1997 Published by Elsevier Inc.
