Abstract
The ability of β-carotene, lutein or astaxanthin to protect against UVA-induced oxidative stress in
rat kidney fibroblasts (NRK) was assessed. Activities of the antioxidant enzymes catalase
(CAT) and superoxide dismutase (SOD), and changes in thiobarbituric acid reactive
substances (TBARS) were measured as indices of oxidative stress. Exposure to UVA light
at a dose intensity of 5.6 mW/cm2 for 4 h resulted in a significant decrease in CAT and SOD activities and a significant
increase in TBARS. No cytotoxicity, as indicated by lactate dehydrogenase (LDH) release,
was observed. β-Carotene (1 μM), lutein (1 μM) and astaxanthin (10 nM) protect against UVA light-induced oxidative stress in vitro
with astaxanthin exhibiting superior protective properties.
Keywords
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References
- Suppression of ultraviolet light-induced tumour formation by dietary antioxidants.J Invest Dermatol. 1975; 65: 412-414
- Carotenoids affect development of UVB-induced skin cancer.Photochem Photobiol. 1987; 46: 507-509
- A clinical trial of beta carotene to prevent basal-cell and squamous cell cancers of the skin.N Engl J Med. 1990; 323: 789-795
- Carotenoids: functions and fallacies.Proc Nutr Soc. 1994; 53: 77-87
Chopra M, Wilson RL, Thurnham DI. Free radical scavenging ability of lutein in vitro. In: Canfield LM, Krinsky NI, Olson JA, editors. Carotenoids in Human Health. New York: Ann NY Acad Sci, 1993:61–7.
- Inhibition of oxidative injury of biological membranes by astaxanthin.Physiol Chem Phys Med NMR. 1990; 22: 27-38
- Astaxanthin and canthaxanthin are potent antioxidants in a membrane model.Arch Biochem Biophys. 1992; 297: 291-295
- Astaxanthin: antioxidant effects in chicken embryo fibroblasts.Nutr Res. 1995; 15: 1695-1704
- Development of an in vitro cell culture model to investigate the induction and quantification of oxidative stress and its inhibition by α-tocopherol.Toxicol In Vitro. 1994; 8: 67-73
- Modulation of oxidative stress by β-carotene in chicken embryo fibroblasts.Br J Nutr. 1995; 73: 841-850
Wilson AM, Sisk RM, O'Brien MN. Modulation of cholestan-3β,5α,6β-triol toxicity by butylated hydroxytoluene, α-tocopherol and β-carotene in newborn rat kidney cells in vitro. Br J Nutr 1997;78:479–92.
Vassault A. Lactate dehydrogenase. In: Bergmeyer HU, editor. Methods of Enzymatic Analysis. New York: Academic Press, 1983:118–26.
- Tissue fractionation studies. Intracellular distribution of monoamine oxidase, aspartate aminotransferase, alanine aminotransferase, d-amino acid oxidase and catalase in rat liver tissue.Biochem J. 1964; 92: 179-184
- Superoxide dismutase. An enzyme function for erythrocuprein (hemocuprein).J Biol Chem. 1969; 244: 6049-6055
Sheehy PJA, O'Mahony E, O'Sullivan-Corbett K, Salvatella M. Simultaneous determination of retinol, α-, γ- and δ-tocopherol, 6 carotenoids and internal standards in plasma by reverse-phase HPLC. FLAIR Meeting, Montreaux, 1994.
- Diverse carotenoids protect against chemically induced neoplastic transformation.Carcinogene. 1991; 12: 671-678
- Ultraviolet light-induced reductions in plasma carotenoid levels.Am J Clin Nutr. 1988; 47: 879-883
- Beta carotene therapy for erythropoietic protoprophyria and other photosensitivity diseases.Arch Dermatol. 1977; 113: 1229-1232
- Carotenoids affect development of UVB induced skin cancer.Photochem Photobiol. 1987; 46: 507-509
- Effect of β-carotene supplementation on photosuppression of delayed-type hypersensitivity in normal young men.Am J Clin Nutr. 1992; 56: 684-690
Article info
Publication history
Accepted:
July 8,
1997
Received in revised form:
June 16,
1997
Received:
April 14,
1997
Identification
Copyright
© 1998 Elsevier Science Ireland Ltd. Published by Elsevier Inc. All rights reserved.
