Advertisement

Effects of various salts and irradiation with UV light on the attachment of Staphylococcus aureus strains

      Abstract

      We investigated the attachment of Staphylococcus aureus isolated from atopic dermatitis lesions to plastic tissue-culture coverslips. We found that attachment was weaker in (rabbit) plasma with 5 or 10% NaCl and in plasma with 5 or 10% sea salts than in the control plasma after incubation for 2 h (P<0.01). The attachment was weaker still in plasma with 10% NaCl or 10% sea salts than in the control plasma after incubation for 24 h (P<0.01). Plasma coagulation of four S. aureus strains isolated from atopic dermatitis lesions was not detected in plasma with 10% NaCl (pH 5.6) or 10% sea salts (containing 0.372% Mg2+) after incubation for 12, 24, 36 and 60 h. The attachment of S. aureus strain cells to the coverslip in plasma was weaker after irradiation with UVA at 25 or 50 J/cm2 (P<0.01) and UVB at 0.5 J/cm2 (P<0.05) both of which are covered by a black cloth, than without irradiation after incubation for 24 h. Plasma coagulation was not detected after irradiation with UVA at 25 or 50 J/cm2 with a black cloth cover (temperature reached 50°C), but was detected after UVA irradiation at the same doses combined with cooling (temperature reached 22°C), after incubation for 24 h. The results suggest that the attachment of S. aureus cells isolated from atopic dermatitis lesions to the coverslip is suppressed in the presence of 10% salts and irradiation with UVA and UVB, and that plasma coagulation of S. aureus cells isolated from atopic dermatitis lesions is suppressed in the presence of 10% salts, irradiation with UVA, and heating.

      Keywords

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Journal of Dermatological Science
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Leyden J.J
        • Marples R.R
        • Kligman A.M
        Staphylococcus aureus in the lesions of atopic dermatitis.
        Br J Dermatol. 1974; 90: 525-530
        • Williams R.E.A
        • Gibson A.G
        • Aitchison T.C
        • Lever R
        • Mackie R.M
        Assessment of a contact-plate sampling technique and subsequent quantitative bacterial studies in atopic dermatitis.
        Br J Dermatol. 1990; 123: 493-501
        • Akiyama H
        • Toi Y
        • Kanzaki H
        • Tada J
        • Arata J
        Prevalence of producers of enterotoxins and toxic shock syndrome toxin-1 among Staphylococcus aureus strains isolated from atopic dermatitis lesions.
        Arch Dermatol Res. 1996; 288: 418-420
        • Akiyama H
        • Ueda M
        • Toi Y
        • Kanzaki H
        • Tada J
        • Arata J
        Comparison of the severity of atopic dermatitis lesions and the density of Staphylococcus aureus on the lesions after antistaphylococcal treatment.
        J Infect Chemother. 1996; 2: 70-74
        • Akiyama H
        • Tada J
        • Toi Y
        • Kanzaki H
        • Arata J
        Changes in Staphylococcus aureus density and lesion severity after topical application of povidone-iodine in cases of atopic dermatitis.
        J Dermatol Sci. 1997; 16: 23-30
        • Tubaki T
        • Iikura Y
        • Saito H
        • Akasawa A
        • Tabana T
        • Matsuda S
        • Koshibu T
        • Iwasaki A
        • Obata T
        • Sugihara Y
        Changes in histamine / tryptase levels in skin chambers: application for clinical evaluation of atopic dermatitis.
        Int Arch Allergy Immunol. 1992; 99: 459-462
        • Akiyama H
        • Ueda M
        • Kanzaki H
        • Tada J
        • Arata J
        Biofilm formation of Staphylococcus aureus strains isolated from impetigo and furuncle: role of fibrinogen and fibrin.
        J Dermatol Sci. 1997; 16: 2-10
        • Kanemasa Y
        • Yoshioka T
        • Hayashi H
        Alteration of the phospholipid composition of Staphylococcus aureus cultured in medium containing NaCl.
        Biochim Biophys Acta. 1972; 280: 444-450
      1. Hayashi H., Koujima I., Kiguchi K., Kanamasa Y. Salt tolerance mechanism of Staphylococcus aureus: energy metabolism at high salinity. Jpn J Bacteriol 1978;33:539–546 (in Japanese).

        • Christian J.H.B
        • Waltho J.A
        The composition of Staphylococcus aureus in relation to the water activity of the growth medium.
        J Gen Microbiol. 1964; 35: 205-213
        • Measures J.C
        Role of amino acids in osmoregulation of non-halophilic bacteria.
        Nature. 1975; 257: 398-400
      2. Busta F.F., Jezeski J.J. Effect of sodium chloride concentration in an agar medium on growth of heat-shocked Staphylococcus aureus. Appl Microbiol 1963;11:404–407.

        • Hurst A
        • Hughes A
        • Beare-Rogers J.L
        • Collins-Thompson D.L
        Physiological studies on the recovery of salt tolerance by Staphylococcus aureus after sublethal heating.
        J Bacteriol. 1973; 116: 901-907
        • Kawabata S
        • Morita T
        • Iwanaga S
        • Igarashi H
        Enzymatic properties of staphylothrombin, an active molecular complex formed between staphylocoagulase and human prothrombin.
        J Biochem. 1985; 98: 1603-1614
        • Soulier J.P
        • Prou O
        • Halle L
        Further studies on thrombin-coagulase.
        Thrombos Diathes Haemorrh. 1970; 23: 37-49
      3. White A., Handler P., Smith E.L., Hill R.L., Lehman I.R. Enzymes I, nature, classification, kinetics, metabolic inhibitors: control of enzymatic activity. In: White A, editor. Principles of Biochemistry. Tokyo: McGraw-Hill, 1978:196–230.

        • Vesterberg K
        Vesterberg O. Studies on staphylokinase.
        J Med Microbiol. 1972; 5: 441-450
        • Davidson F.M
        The activation of plasminogen by staphylokinase: comparison with streptokinase.
        Biochem J. 1960; 76: 56-61
        • Kanzaki H
        • Morishita Y
        • Akiyama H
        • Arata J
        Adhesion of Staphylococcus aureus to horny layer: role of fibrinogen.
        J Dermatol Sci. 1996; 12: 132-139
        • Jekler J
        • Larkö O
        UVB phototherapy of atopic dermatitis.
        Br J Dermatol. 1988; 119: 697-705
        • Jekler J
        • Larkö O
        Combined UVA-UVB versus UVB phototherapy for atopic dermatitis: a paired-comparison study.
        J Am Acad Dermatol. 1990; 22: 49-53
        • Jekler J
        • Larkö O
        UVA solarium versus UVB phototherapy of atopic dermatitis: a paired-comparison study.
        Br J Dermatol. 1990; 125: 569-572
        • Faergemann J
        • Larkö O
        The effect of UV-light on human skin microorganisms.
        Acta Derm Venereol (Stockh). 1987; 67: 69-72
        • Jekler J
        • Bergbrant I.-M
        • Faergemann J
        Larkö 0. The in vivo effect of UVB radiation on skin bacteria in patients with atopic dermatitis.
        Acta Derm Venereol (Stockh). 1992; 72: 33-36
        • Yoshimura M
        • Namura S
        • Akamatsu H
        • Horio T
        Antimicrobial effects of phototherapy and photochemotherapy in vive end in vitro.
        Br J Dermatol. 1996; 135: 528-532
      4. Akiyama H., Tada J., Makino H., Torigoe R., Abe Y., Toi Y., Morishita Y., Shimoe K., Kanzaki H., Arata J., Nagao Y., Yamada T., Katayama H., Asagoe K. Staphylococcus aureus isolates from skin lesions, nostrils, and ears of impetigo contagiosa patients with or without atopic dermatitis. Jap J Dermatol 1994;104:655–661 (in Japanese).