Abstract
We investigated the attachment of Staphylococcus aureus isolated from atopic dermatitis lesions to plastic tissue-culture coverslips. We
found that attachment was weaker in (rabbit) plasma with 5 or 10% NaCl and in plasma
with 5 or 10% sea salts than in the control plasma after incubation for 2 h (P<0.01). The attachment was weaker still in plasma with 10% NaCl or 10% sea salts than
in the control plasma after incubation for 24 h (P<0.01). Plasma coagulation of four S. aureus strains isolated from atopic dermatitis lesions was not detected in plasma with 10%
NaCl (pH 5.6) or 10% sea salts (containing 0.372% Mg2+) after incubation for 12, 24, 36 and 60 h. The attachment of S. aureus strain cells to the coverslip in plasma was weaker after irradiation with UVA at
25 or 50 J/cm2 (P<0.01) and UVB at 0.5 J/cm2 (P<0.05) both of which are covered by a black cloth, than without irradiation after
incubation for 24 h. Plasma coagulation was not detected after irradiation with UVA
at 25 or 50 J/cm2 with a black cloth cover (temperature reached 50°C), but was detected after UVA irradiation
at the same doses combined with cooling (temperature reached 22°C), after incubation
for 24 h. The results suggest that the attachment of S. aureus cells isolated from atopic dermatitis lesions to the coverslip is suppressed in the
presence of 10% salts and irradiation with UVA and UVB, and that plasma coagulation
of S. aureus cells isolated from atopic dermatitis lesions is suppressed in the presence of 10%
salts, irradiation with UVA, and heating.
Keywords
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Article info
Publication history
Accepted:
June 26,
1997
Received in revised form:
June 17,
1997
Received:
April 15,
1997
Identification
Copyright
© 1998 Elsevier Science Ireland Ltd. Published by Elsevier Inc. All rights reserved.
