Abstract
In order to know the possible effects of gastrin releasing peptide (GRP) on nevus
cells and melanocytes, we studied the effect of GRP on the proliferation of cultured
human nevus cells and normal melanocytes. MTS assay showed that GRP stimulated the
growth of viable melanocytes at 1000 ng/ml. GRP also stimulated the growth of nevus
cells in a dose dependent manner and maximum stimulation was obtained at 100 ng/ml
of GRP. GRP was less effective for growth stimulation of normal melanocytes than nevus
cells. The cytoplasm of nevus cells were positively stained by polyclonal anti-GRP
antibody. We also detected the expression of GRP and GRP receptor mRNAs in these cells
by RT-PCR. These results suggest that GRP acts as an autocrine growth factor for nevus
cells and normal melanocytes.
Keywords
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Journal of Dermatological ScienceAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- A gastrin releasing peptide from the porcine nonantral gastric tissue.Gut. 1978; 19: 767-774
- Isolation and structure of bombesin and alytesin, two analogous active peptides from the skin of the europian amphibians Bombina and Alytes.Experientia. 1971; 27: 166-167
- Characterization of s gastrin releasing peptide from porcine non-antral gastric tissue.Biochem Biophys Res Commun. 1979; 90: 227-233
- Stimulating effect of bombesin on the growth of gastrointestinal tract and pancreas in suckling rats.Gastroenterology. 1986; 90: 1942-1949
- Transient elevation of messenger RNA encoding gastrin-releasing peptide, a putative pulmonary growth factor in human fetal lung.J Clin Invest. 1987; 80: 1172-1179
Takeyama M, Kino T, Kondo K, Guo LL, Otaka A, Fujii N, Yajima H. Immuno-affinity purification of specific antibodies against human gastrin releasing peptide (h-GRP) by the h-GRP (1-8)-linked polydimethylacrylamide resin. Int J Peptide Prot Res 1989a;33:457–467; Takeyama M, Kondo K, Hayashi Y, Yajima H. Enzyme immunoassay of gastrin releasing peptide (GRP)-like immunoreactivity in milk. Int J Peptide Prot. Res 1989b;34:70–74.
- Gastrin releasing peptide-like immunoreactive substance in rat mammary glands during pregnancy.Biochem Biophys Res Comm. 1991; 180: 455-461
- Mitogenic action of gastrinreleasing polypeptide on isolated epiphyseal growth plate chondrocytes from the ovine fetus.Endocrinology. 1992; 130: 2811-2819
- Distribution of a bombesin-like peptide in human gastrointestinal tract.Lancet. 1976; 1: 1109-1110
- Bombesin-like peptides in mammals.Fed Proc. 1979; 38: 2315-2319
- Bombesin-like immunoreactivity in the lung.Nature. 1978; 273: 769-770
- Dose-response comparisons of canine plasma gastroenteropancreatic hormone responses to bombesin and the porcine gastrin-releasing peptide (GRP).Regul Pept. 1983; 5: 125-137
- The role of vagal integrity in gastrin releasing peptide stimulated gastroenteropancreatic hormone release and gastric acid serection.Regul Pept. 1985; 10: 179-187
- Gut hormones in gastric function.Baillieres Clin Endocrinol Metab. 1994; 8: 111-136
- Bombesin-like activity. Radioimmunologic assessment in biological tissues.Life Sci. 1978; 23: 2721-2728
- Bombesinlike peptides in rat brain. Localization in synaptosomes and release from hypothalamic slices.Life Sci. 1980; 26: 1707-1712
- Bombesin stimulates prolactin and growth hormone release by pituitary cells in culture.Endocrinology. 1982; 110: 352-358
- Active polypeptides of the amphibian skin and their synthetic analogues.Pure Appl Chem. 1973; 35: 463-494
- Bombesin stimulation of DNA synthesis and cell division in culture of Swiss 3T3 cells.Proc Natl Acad Sci USA. 1983; 80: 2936-2940
- Role of Ca2+ influx in bombesin-induced mitogenesis in Swiss 3T3 fibroblasts.J Biol Chem. 1991; 266: 1403-1409
- Bombesin and the C-terminal tetrodecapeptide of gastrin-releasing peptide are growth factors for normal human bronchial epithlial cells.Exp Cell Res. 1984; 153: 245-248
- Bombesin stimulates growth of human prostatic cancer cells in vitro.Cancer. 1989; 63: 1714-1720
- Selective stimulation of small cell lung cancer clonal growth by bombesin and gastrin-releasing peptide.Cancer Res. 1987; 47: 821-825
- Bombesin-like peptides can function as autocrine growth factors in human small-cell lung cancer.Nature. 1985; 316: 823-827
- Expression of cytokine/growth factors and their receptors in human melanoma and melanocytes.Int J Cancer. 1994; 56: 853-857
- Innervation of melanocytes in human skin.J Exp Med. 1996; 184: 1385-1395
- Characteristics of cultivated adult human nevocellular nevus cells.J Invest Dermatol. 1986; 87: 102-107
- Serum-free culture of normal human melanocytes. Growth kinetics and growth factor requirements.J Cell Physiol. 1989; 140: 565-576
- Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture.Cancer Commun. 1991; 3: 207-212
- Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.Analyt Biochem. 1987; 162: 156-159
- Cloning and characterization of cDNAs encoding human gastrin-releasing peptide.Proc Natl Acad Sci USA. 1984; 81: 5699-5703
- Two distinct bombesin receptor subtypes are expressed and functional in human lung carcinoma cells.J Biol Chem. 1991; 266: 18771-18779
- Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs. Genomic complexity and molecular evolution of the gene.Nucleic Acids Res. 1985; 13: 2485-2502
- Neuropeptides as cellular growth factors. Role of multiple signalling pathways.Eur J Clin Invest. 1991; 21: 123-134
- Neuropeptide growth factors and cancer.Br J Cancer. 1991; 63: 469-475
- Neuropeptide growth factors.Cancer Invest. 1991; 9: 659-673
- The effect of bombesin on polyphosphoinositide and calcium metabolism in Swiss 3T3 cells.J Biol Chem. 1987; 262: 182-188
- Studies and perspectives of protein kinase C.Science. 1986; 18: 305-312
- Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin.Proc Natl Acad Sci USA. 1982; 79: 2018-2022
Article info
Publication history
Accepted:
November 18,
1997
Received in revised form:
September 24,
1997
Received:
August 16,
1997
Identification
Copyright
© 1997 Elsevier Science Ireland Ltd. Published by Elsevier Inc. All rights reserved.
