Abstract
In this report, we applied the isoleucine-deprivation method to normal human keratinocytes
in cultivation with serum-free MCDB153 medium. The growing cells were synchronized
by transfer to MCDB153 medium prepared without isoleucine, and the degree of synchronization
was analyzed by the cell cycle distribution and phosphorylation of retinoblastoma
protein (pRB), one of the tumor suppressor gene products. 1,25-dihidroxyvitamin D3 (1,25(OH)2D3), a biologically active form of vitamin D3, is supposed to induce the differentiation and growth inhibition of human keratinocytes
caused by cell cycle arrest. We examined its effect on cell cycle kinetics, especially
progression from G1/G0 to S-phase, by using this synchronized system. We showed that
10−6 M 1,25(OH)2D3 inhibited the progression from G1/G0 to S-phase strikingly in synchronized normal
human keratinocytes.
Keywords
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Article info
Publication history
Accepted:
October 17,
1997
Received in revised form:
September 18,
1997
Received:
May 7,
1997
Identification
Copyright
© 1998 Elsevier Science Ireland Ltd. Published by Elsevier Inc. All rights reserved.
