Abstract
A human melanoma-associated antigen D-1 was recently identified by screening an expression
cDNA library derived from mRNA of cultured melanoma cells with sera of melanoma patients.
The aim of this study is to present in vivo expression and precise distribution of
D-1 in normal tissues and benign or malignant neoplasms. By in situ hybridization,
we found that the D-1 mRNA was exclusively expressed in the cytoplasms of melanoma
cells, but not in keratinocytes, fibroblasts and lymphocytes adjacent to melanoma
nests. Further immunohistochemical studies revealed that the expression of D-1 antigen
was distributed to both the surface and cytoplasm of melanoma cells, indicating that
D-1 antigen can be recognized by killer T lymphocytes or antibodies in vivo. No significant
mRNA nor peptide of D-1 was detected in basal cell carcinoma, squamous cell carcinoma
and other benign tumors such as melanocytic nevi and seborrheic keratosis. We also
confirmed that D-1 mRNA and peptide were not expressed in normal organs by dot blot
hybridization and western blot analysis, respectively. These results will assess the
suitability of recombinant D-1 protein to implement active specific immunotherapy
against melanoma.
Keywords
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Journal of Dermatological ScienceAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Cutaneous melanoma.New Engl J Med. 1989; 325: 171-182
- Antibody response and tumor growth in syngeneic mice immunized to partially purified B16 melanoma-associated antigens.J Immunol. 1978; 120: 96-101
- Induction of cell-mediated immunity to autologous melanoma cells and regression of metastases after treatment with a melanoma cell vaccine preceded by cyclophosphamide.Cancer Res. 1986; 6: 2572-2577
- Vaccines containing purified GM2 ganglioside elicit GM2 antibodies in melanoma patients.Proc Natl Acad Sci USA. 1987; 84: 2911-2915
- Active specific immunotherapy in patients with melanoma: a clinical trial with mouse antiidiotypic monoclonal antibodies elicited with syngeneic anti-high-molecular-weight-melanoma-associated antigen monoclonal antibodies.J Clin Invest. 1990; 86: 2136-2144
- Human high molecular weight melanoma-associated antigen (HMW-MAA) mimicry by mouse anti-idiotypic monoclonal antibody: induction of humoral anti-HMW-MAA immunity and prolongation of survival in patients with stage melanoma.Proc Natl Acad Sci USA. 1992; 89: 466-470
- Cloning and in vitro expression of a melanoma-associated antigen immunogenic in patients with melanoma.J Immunol. 1991; 147: 1098-1104
- Non-radioactive labeling of RNA transcripts in vitro with the hapten digoxigenin (DIG); hybridization and ELISA-based detection.Nucleic Acids Res. 1990; 118: 5843-5851
- Localization of a family of mRNAs in a single cell type and its precursors in sea urchin embryos.Proc Natl Acad Sci USA. 1983; 80: 2656-2660
- Localization of mRNA for C-kit receptor and its ligand in the brain of adult rats: an analysis using in situ hybridization.Mol Brain Res. 1992; 15: 47-54
Sambrook J, Fritsch EF, Maniatis T. In: Molecular Cloning, A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory 1989;1:21–27.
- Double determinant immunoassay to measure a human high-molecular-weight melanoma-associated antigen.Cancer Res. 1983; 43: 3586-3590
- Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.Biochemistry. 1979; 18: 5294-5299
Sambrook J, Fritsch EF, Maniatis T. In: Molecular Cloning, A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory 1989;7:53–57.
Article info
Publication history
Accepted:
December 10,
1997
Received in revised form:
November 28,
1997
Received:
October 24,
1997
Identification
Copyright
© 1998 Elsevier Science Ireland Ltd. Published by Elsevier Inc. All rights reserved.
