Abstract
We have developed two different novel immunoprecipitation assays in which radioisotopes
are not used, and have examined antigens for four cases of paraneoplastic pemphigus
(PNP) including three new patients. The PNP sera showed a clear reactivity with transitional
epithelia of rat urinary bladder by immunofluorescence, and reacted with a characteristic
doublet of the 210 and 190 kD proteins by immunoblotting of normal human epidermal
extract, confirming the diagnosis of PNP. In addition, by immunoprecipitation using
silver-stain to detect immunoprecipitated proteins, the PNP sera detected the 250,
210 and 190 kD proteins, while control bullous pemphigoid sera detected only the 230
kD bullous pemphigoid antigen. Furthermore, with another immunoprecipitation using
cell surface biotinylation, three of the four PNP sera specifically reacted with the
130 kD pemphigus vulgaris antigen (Dsg3), indicating that pemphigus vulgaris antigen
may be involved in PNP. This reactivity was further suggested by the immunoblot analysis
using recombinant pemphigus vulgaris antigen. In future, these non-radioisotope immunoprecipitation
assays should become a useful tool not only to unravel the complex situation for the
PNP antigens, but also to study antigens in other autoimmune bullous skin diseases.
Keywords
Abbreviations:
BMZ, basement membrane zone (), BP, bullous pemphigoid (), IB, immunoblotting (), IIF, indirect immunofluorescence (), IP, immunoprecipitation (), mAb, monoclonal antibody (), PF, pemphigus foliaceus (), PNP, paraneoplastic pemphigus (), PV, pemphigus vulgaris ()To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
January 16,
1998
Received in revised form:
January 13,
1998
Received:
July 22,
1997
Identification
Copyright
© 1998 Elsevier Science Ireland Ltd. Published by Elsevier Inc. All rights reserved.
