Apoptosis is a biochemically and morphologically gene-regulated distinctive form of cell death playing a pivotal role in tissue homeostatis, viral infections and clearance of damaged cells. The process is initiated by a cascade of intercellular and intracellular signals through an intrinsic cell suicide program resulting in early DNA fragmentation characterized by nuclear and cytoplasmic condensation. Recently some authors have reported apoptosis to occur in several inflammatory skin diseases, such as lichenoid reactions and cutaneous lymphomas. The aim of our study is to investigate the apoptotic phenomenon in two different forms of cutaneous necrotizing vasculitis (CNV) affecting the postcapillary venules such as leukocytoclastic and lymphocytic cutaneous vasculitis. For this purpose, the in situ nick end labelling of fragmented DNA technique has been performed on lesional skin biopsies from patients with acute phase of the disease. In both leukocytoclastic and lymphocytic forms apoptotic bodies were detected, evidencing two different characteristic patterns of distribution, probably related to the different nature of cellular inflammatory infiltrate. Our results seem to account for the involvement of apoptotic phenomena in cutaneous vasculitis; furthermore, the evaluation of in situ DNA fragmentation could be a useful tool to discriminate different forms of the disease.
To read this article in full you will need to make a payment
Purchase one-time access:Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
One-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:Subscribe to Journal of Dermatological Science
Already a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
Ryan TJ. Cutaneous vasculitis. In: Rook A, Wilkinson DS, Ebling FJB, editors. Textbook of Dermatology, 5th ed. Oxford: Blackwell, 1992.
- The natural history of vasculitis.Arch Dermatol. 1990; 126: 84-89
- Apoptosis, oncosis and necrosis.Am J Pathol. 1995; 146: 3-15
- : Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics.Br J Cancer. 1972; 26: 239-257
- Apoptosis: its nature and implications for dermatopathology.Am J Dermatopathol. 1979; 1: 133-144
- Patterns of cell death: the significance of apoptosis for dermatology.Exp Dermatol. 1993; 2: 3-11
- Apoptotic bodies and abnormally dividing epithelial cells in squamous cell carcinoma.Histopathology. 1986; 10: 921-931
- Endogenous endonuclease-induced DNA fragmentation: an early event in cell-mediated cytolysis.Proc Natl Acad Sci USA. 1983; 80: 6361-6395
- Cell death mechanism and the immune system.Immunol Rev. 1991; 121: 29-35
- Dendrocytoclasis in Henoch-Schonlein Purpura.Acta Derm Venereol. 1991; 71: 358-359
- Apoptotic cell death of neutrophils in development of skin lesions of patients with Anaphylactoid purpura.J Dermatol. 1997; 24: 94-99
Accepted: January 23, 1998
Received in revised form: December 22, 1997
Received: August 4, 1997
© 1998 Elsevier Science Ireland Ltd. Published by Elsevier Inc. All rights reserved.