Abstract
Reactive oxygen species (ROS) play a role in the modulation of apoptosis. Antioxidant
defence mechanisms against cell death involving apoptosis due to UVB irradiation were
studied on three established cell lines (SCC derived from human skin squamous cell
carcinoma, F-SV and F-ST derived from human skin fibroblasts) which were susceptible
to cell death by UVB irradiation (12.5–250 mJ/cm2), and one cell line (N-F) derived from primary cultured human skin fibroblasts which
was resistant to cell death. We compared antioxidant defences between the three established
cell lines and N-F, measuring four antioxidant enzymes (superoxide dismutase (SOD),
catalase, glutathione peroxidase (GSH-Px) and glutathione reductase (GR)) and a non-enzymatic
antioxidant glutathione. The greatest difference was that Cu, Zn-SOD activity in N-F
was 3–4-times the three other cell lines. Though SCC had much larger amounts of glutathione
and higher antioxidant enzyme activities except for Cu, Zn-SOD than N-F, SCC was very
susceptible to cell death. After UVB irradiation (at 16 h after 12.5 mJ/cm2), in all cell lines, SOD activity increased 1.1–1.3-times that of non-irradiated
cells, while other enzyme activities remained constant. This presumably represents
a protective response against ROS generated during UVB irradiation. N-F which was
resistant to UVB-induced cell death had higher Cu, Zn-SOD activity before UVB irradiation,
and a larger increase of SOD (mainly Mn-SOD) after UVB exposure than the other cell
lines which were susceptible to cell death. Therefore, we conclude that the most important
enzymatic antioxidant to protect cells from UVB damage is SOD.
Keywords
Abbreviations:
BHT, butylated hydroxytoluene (), BSO, buthionine sulfoximine (), ECL, established cell line (), GR, glutathione reductase (), GSH-Px, glutathione peroxidase (), PDTC, pyrrolidine dithiocarbamate (), ROS, reactive oxygen species (), SOD, superoxide dismutase (), TBARS, thiobarbituric acid reactive substances (), UV, ultraviolet ray ()To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
December 26,
1997
Received in revised form:
November 26,
1997
Received:
September 2,
1997
Identification
Copyright
© 1998 Elsevier Science Ireland Ltd. Published by Elsevier Inc. All rights reserved.
