Abstract
We investigated the photoprotective effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) both in vivo and in vitro, revealing its relationship with glutathione, a well-known
antioxidant. We also probed into the possible mechanism of photoprotection of 1,25(OH)2D3 through immunohistochemical study for metallothionein (MT). At the same time, endogenous
antioxidant effect of 1,25(OH)2D3 was examined. Survival of cultured human keratinocytes was decreased when the cells
were irradiated with ultraviolet light-B (UVB) at doses above 30 mJ/cm2. But in the presence of 1,25(OH)2D3 (12 nM), the decrease of survival of keratinocytes by UVB was diminished. The formation
of sunburn cells by UVB irradiation in the skin of ICR mice was inhibited by topical
application of 1,25(OH)2D3, regardless of prior glutathione depletion. Immunohistochemical staining revealed
that 1,25(OH)2D3 induced the expression of MT, a potent radical scavenger, mainly in the basal layer
of ICR mice skin. 1,25(OH)2D3 neither inhibited peroxidation of plasma lipids nor interacted with superoxide, nor
removed hydrogen peroxide as an antioxidant. These findings suggest that 1,25(OH)2D3 has photoprotective effect not related with glutathione or its endogenous antioxidant
property. Rather, it could be attributed to 1,25(OH)2D3-induced MT and its capacity to prevent radical-related damage in UVB irradiation.
Keywords
Abbreviations:
BSO, DL-buthionine-[S,R]-sulfoximine (), MT, metallothionein (), MTT test, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrasoliumbromide colorimetric assay (), SBC, sunburn cell (), UVB, ultraviolet light B (), 1,25(OH)2D3, 1,25-dihydroxyvitamin D3 ()To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
January 21,
1998
Received in revised form:
December 24,
1997
Received:
November 4,
1997
Identification
Copyright
© 1998 Elsevier Science Ireland Ltd. Published by Elsevier Inc. All rights reserved.
