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Sensitivity of cross-reacting antihuman antibodies in formalin-fixed porcine skin: including antibodies to proliferation antigens and cytokeratins with specificity in the skin1

      Abstract

      Although no animal is a perfect skin model for the study of toxicological and therapeutic agents, structurally the pig may be superior to even non-human primates. Because our work involves effects of toxicological and therapeutic agents on the skin, we wanted to identify stains which may prove useful as well as determine cross-reactivity of some newer antihuman antibodies. We performed a battery of formalin-fixed skin from weanling pigs and minipigs. The battery of antibodies included LCA, CD3, OPD-4, CD34, UCHL-1, L-26, KP-1, MAC-387, Factor XIIIa, Leu-7, S-100 protein, HMB-45, GFAP, synaptophysin, neurofilament protein, ubiquitin, vimentin, type IV collagen, laminin, fibronectin, Factor VIII related antigen, Desmin-M, smooth muscle actin, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, EMA, GCDFP, Ki-67, and PCNA. Immunohistochemical stains for CD3, Leu-7, S-100 protein, type IV collagen, laminin, Factor VIII related antigen, GFAP, synaptophysin, neurofilament protein, ubiquitin, smooth muscle actin, vimentin, Desmin-M, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, Ki-67 and PCNA showed consistent cross-reactivity. In formalin-fixed tissue, only antibodies to lymphoreticular cells showed poor cross-reactivity. A high percentage of the remaining antibodies did show good cross-reactivity but with some interesting similarities and differences in specificity.

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      References

        • Bartek M.J
        • LaBudde J.A
        • Maiback H.I
        Skin permeability in vivo: Comparison in rat, rabbit, pig, and man.
        J Invest Dermatol. 1972; 58: 114-123
        • Braverman I.M
        Ultrastructure and organization of the cutaneous microvasculature in normal and pathologic states.
        J Invest Dermatol. 1989; 93: 2S-9S
        • Dick I.P
        • Scott R.C
        Pig ear skin as an in-vitro model for human skin permeability.
        J Pharm Pharmacol. 1992; 44: 640-645
        • Gailit J
        • Welch M.P
        • Clark R.A.F
        TGF-beta1 stimulates expression of keratinocyte integrins during re-epithelialization of cutaneous wounds.
        J Invest Dermatol. 1994; 103: 221-227
      1. Geary WA, Cooper PH. Proliferating cell nuclear antigen (PCNA) in common epidermal lesions. 1991;19:458–468.

        • Higgins J.C
        • Eady R.A.J
        Human dermal microvasculature: A morphological and enzyme histochemical investigation at the light and electron microscope levels.
        Br J Dermatol. 1981; 104: 117-129
        • Hochstrasser M
        Ubiquitin and intracellular protein degradation.
        Curr Opin Cell Biol. 1992; 4: 1024-1031
        • Horiguchi Y
        • Maruguchi T
        • Maruguchi Y
        • Suzuki S
        • Fine J.D
        • Leigh I.M
        • Yoshiki T
        • Uedda M
        • Toda K.I
        • Isshiki N
        Ultrastructural and immunohistochemical characterization of basal cells in three-dimensional culture models of the skin.
        Arch Dermatol Res. 1994; 286: 53-61
      2. Woodward DF, Nieves AL, Williams LS, Spada CS, Hawley, Duenes. A new hairless strain of guinea pig: Characterization of the cutaneous morphology and pharmacology. In: Maibach HI, Lowe NJ, editors. Models of Dermatology, vol. 4. Basel: Karger, 1989:71–78.

        • Lavker R.M
        • Dong G
        • Zheng P
        • Murphy G.F
        Hairless micropig skin: A novel model for studies of cutaneous biology.
        Am J Pathol. 1991; 138: 687-697
        • Lavker R.M
        • Sun T.T
        Hair follicle stem cells: present concept.
        J Invest Dermatol. 1995; 104: 38S-39S
        • Mackenzie I.C
        Ordered structure of the epidermis.
        J Invest Dermatol. 1975; 65: 45-51
        • Mann S.J
        Varieties of hairless-like mutant mice.
        J Invest Dermatol. 1971; 56: 170-173
        • Meyer W
        • Gorgen S
        • Schlesinger C
        Structural and histochemical aspects of epidermis development of fetal porcine skin.
        Am J Anat. 1986; 176: 207-219
        • Meyer W
        • Schwarz R
        • Neurand K
        The skin of domestic mammals as a model for the human skin, with special reference to the domestic pig.
        Curr Probl Dermatol. 1978; 7: 39-52
      3. Montagna W. The epidermis. In: Montagna W, editor. The Structure and Function of the Skin. New York: Academic Press, 1962:19–35.

      4. Montagna W. The dermis. In: Montagna W, editor. The Structure and Function of Skin. New York: Academic Press, 1962:122–124.

        • Montagna W
        • Chase H.B
        • Melaragno H
        The skin of hairless mice: The formation of cysts and the distribution of lipids.
        J Invest Dermatol. 1981; 19: 83-94
        • Montagna W
        • Yun J.S
        The skin of the domestic pig.
        J Invest Dermatol. 1964; 43: 11-21
      5. Monteiro-Riviere NA. Ultrastructural evaluation of the porcine integument. In: Tumbleson ME, editor. Swine in Biomedical Research. New York: Plenum, 1986:651–655.

      6. Monteiro-Riviere MA, Inman AO. Histochemical distribution of five epidermal-dermal junction epitopes in porcine skin treated with Bis (2-Chloroethyl) sulfide. Society of Toxicology, March, 1995.

        • Monteiro-Riviere N.A
        • Stromberg M.W
        Ultrastructure of the integument of the domestic pig (Sus scrofa) through fourteen weeks of age.
        Anat Histol Embryol. 1985; 14: 97-115
        • Parenteau N.L
        • Nolte C.M
        • Bilbo P
        • Rosenberg M
        • et al.
        Epidermis generated in vitro: Practical considerations and applications.
        J Cell Biochem. 1991; 45: 245-251
        • Penneys N.S
        Immunohistochemistry of adnexal neoplasms.
        J Cutaneous Pathol. 1984; 11: 357-364
        • Rigal C
        • Pieraggi M.-T
        • Vincent C
        • Prost C
        • Bouissou H
        • Serre G
        Healing of full-thickness cutaneous wounds in the pig. I. Immunohistochemical study of epidermodermal junction regeneration.
        J Invest Dermatol. 1991; 96: 777-785
        • Smith K.J
        • Graham J.S
        • Skelton H.G
        • O'Leary T
        • Moeller R.B
        • Okerberg C.V
        • Hurst C.G
        Evaluation of the cross-reacting anti-human antibodies in the euthymic hairless guinea pig model.
        Invest J Dermatol Sci. 1997; 14: 240-250
        • Smola H
        • Thiekotter G
        • Fusenig N.E
        Mutual induction of growth factor gene expression by epidermal-dermal cell interaction.
        J Cell Biol. 1993; 122: 417-429
        • Smoller B.R
        • McNutt N.S
        • Hsu A
        HMB-45 recognizes stimulated melanocytes.
        J Cutaneous Pathol. 1989; 16: 49-53
      7. Spearman RIC. The comparative biology of collagenous tissues. In: Jarrett A, editor. The Physiology and Pathophysiology of the Skin. London: Academic Press, 1974:896–899.

        • Wollina U
        • Berger U
        • Mahrle G
        Immunohistochemistry of porcine skin.
        Acta Histochem. 1991; 90: 87-91
      8. Zhang Z, Peters BP, Monteiro-Riviere NA. Assessment of the cutaneous basement membrane in sulfur mustard-induced toxicity. International Symposium on Epidermolysis Bullosa. The University of North Carolina at Chapel Hill, April 26, 1994.