Abstract
Histamine is present in the epidermis in intracellular and extracellular area and
is released from mast cells and keratinocytes in the early stage of inflammation of
the skin. Such release may contribute to common itching or intensify the inflammatory
responses. Histamine binds to its receptors and participate in regulation of the inflammatory
responses by acting on endothelial cells, nerve endings, lymphocytes, monocytes, and
leukocytes. Histamine has direct effects on keratinocytes as well. Histamine modulates
the proliferation of keratinocytes. The binding of histamine to the receptor on keratinocyte
membrane induces activation of adenylate cyclase and phospholipase C through GTP binding
protein. We previously reported that histamine induces transient increase in intracellular
Ca2+ in cultured normal human epidermal keratinocytes (NHEK) and normal epidermis. H1
and H2 histamine receptors are widely distributed in many tissues and cells. In this
study, we investigated which types of histamine receptors are related to the increase
in intracellular Ca2+ by histamine stimulation in cultured human epidermal keratinocytes. NHEK were cultured
in serum-free KGM medium. With H1 antihistamines, mepyramine and diphenhydramine,
histamine responses were moderately but not statistically significantly inhibited.
With H2 antihistamine, cimetidine, histamine response was significantly inhibited.
Epinephrine response was not affected by these antihistamines. Thus, it is considered
that H2 antihistamines specifically block histamine-mediated increase in intracellular
Ca2+ of cultured normal human keratinocytes.
Keywords
Abbreviations:
ICAM-1, intercellular adhesion molecule-1 (), NHEK, normal human epidermal keratinocytes (), PG, prostaglandin (), UV, ultraviolet light ()To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Journal of Dermatological ScienceAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Quantitative measurement of extracellular histamine concentrations in intact human skin in vivo by the microdialysis technique: methodological aspects.Allergy. 1997; 52: 547-555
- Enzymatic histamine degradation by human skin.Br. J. Pharmacol. 1977; 60: 583-587
- Cis-urocanic acid synergizes with histamine for increased PGE2 production by human keratinocytes: link to indomethacin-inhibitable UVB-induced immunosuppression.Photochem. Photobiol. 1995; 61: 303-309
- Effect of histamine receptor antagonists on immunosuppression induced by the cis-isomer of urocanic acid.Photodermatol. Photoimmunol. Photomed. 1990; 7: 243-248
- Histamine in human epidermal cells is induced by ultraviolet light injury.J. Invest. Dermatol. 1996; 106: 785-789
- Histaminergic regulation of antibody-dependent cellular cytotoxicity of granulocytes, monocytes and natural killer cells.J. Leukocyte Biol. 1994; 55: 392-397
- Effect of pharmacologic agents on human mitosis in vitro. III. Inhibition by histamine and methylated analogs.J. Invest. Dermatol. 1975; 65: 400-403
- In vitro analysis of the control of keratinocyte proliferation in human epidermis by physiologic and pharmacologic agents.J. Invest. Dermatol. 1975; 65: 52-59
- Cis-urocanic acid attenuates histamine receptor-mediated activation of adenylate cyclase and increase of intracellular Ca2+.Arch. Dermatol. Res. 1998; 290: 264-269
- Control of epidermal cell proliferation in vitro.Br. J. Dermatol. 1984; 111 Suppl. 27: 18-26]
- The effect of histamine on epidermal outgrowth: its possible dual role as an inhibitor and stimulator.J. Invest. Dermatol. 1981; 76: 24-27
- Histamine and epidermal proliferation.Br. J. Dermatol. 1982; 107: 15-20
- The mast cell products histamine and serotonin stimulate and TNF-alpha inhibits the proliferation of murine epidermal keratinocytes in situ.J. Dermatol. Sci. 1997; 16: 79-84
- Specific refractoriness of adenylate cyclase in skin to epinephrine, prostaglandin E, histamine and AMP.Biochimi. Biophys. Acta. 1977; 497: 428-436
- Histamine (H2) receptor-adenylate cyclase system in pig skin (epidermis).Biochim. Biophys. Acta. 1976; 437: 150-157
- Agonist-induced hydrolysis of phosphoinositides and formation of 1,2-diacylglycerol in adult human keratinocytes.J. Invest. Dermatol. 1989; 93: 241-245
- Adenylate cyclase induces intracellular calcium increase in single human epidermal keratinocytes measured by fluorescence microscopy using Fura 2-AM.Br. J. Dermatol. 1992; 127: 589-594
- Adenylate cyclase induces intracellular Ca2+ increase in single human epidermal keratinocytes of the epidermal sheet as measured by digital imaging microscopy using Fura 2-AM.Arch. Dermatol. Res. 1991; 283: 91-95
- The third histamine receptor. Highly potent and selective ligands.Nature. 1987; 327: 117-123
- Functional diversity of histamine and histamine receptors.J. Invest. Dermatol. 1992; 98: 8S-11S
- Stimulation of in vivo tumor growth and phorbol ester-induced inflammation by N,N-diethyl-2-[4-(phenylmethyl)phenoxy] ethanamine HCl, a potent ligand for intracellular histamine receptors.Biochem. Biophys. Res. Commun. 1991; 179: 1297-1304
- Beta-adrenergic stimulation induces intracellular Ca++ increase in human epidermal keratinocytes.J. Invest. Dermatol. 1991; 96: 234-237
- Regulation of transmembrane signalling system during senescence of human epidermal keratinocytes.Arch. Dermatol. Res. 1996; 288: 611-614
- Interference with the distribution and release of arachidonic acid in human keratinocytes by bradykinin, histamine and phosphatidic acid.Prostaglandins Leukotrienes Med. 1987; 29: 19-23
- Atypical association of H1 and H2 histamine receptors with signal transduction pathways during multistage mouse skin carcinogenesis.Inflamm. Res. 1997; 46: 292-298
- Enhanced prostaglandin synthesis after ultraviolet injury is mediated by endogenous histamine stimulation. A mechanism for irradiation erythema.J. Clin. Invest. 1990; 86: 566-574
- Histamine and cis-urocanic acid augment tumor necrosis factor-alpha mediated induction of keratinocyte intercellular adhesion molecule-1 expression.J. Cell. Physiol. 1993; 156: 348-357
- Cetirizine and hydrocortisone differentially regulate ICAM-1 expression and chemokine release in cultured human keratinocytes.Clin. Exp. Allergy. 1998; 28: 101-109
Article info
Publication history
Accepted:
January 20,
1999
Received:
December 10,
1998
Identification
Copyright
© 1999 Elsevier Science Ireland Ltd. Published by Elsevier Inc. All rights reserved.
